Intact Parathormone (I-PTH) ELISA Kit for Rat

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated I-PTH concentrations in Rat serum, plasma and other body fluids. Using Purified Rat I-PTH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add I-PTH and I-PTH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of I-PTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of I-PTH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS046702 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Intact Parathormone (I-PTH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing I-PTH. The ELISA analytical biochemical technique of the MBS046702 kit is based on I-PTH antibody-I-PTH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect I-PTH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, I-PTH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).