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KLK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468001

Background: Prostate specific antigen (PSA) belongs to the kallikrein gene family. It is a single-chain glycoprotein with a molecular mass of about 33 kD which is produced by prostate epithelial cells. It is secreted in the seminal plasma and is responsible… More details

$615 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PSA (KLK3), Monoclonal Antibody
Full Product Name
anti-human Prostate-Specific Antigen (PSA) monoclonal antibody
Product Gene Name
anti-KLK3 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT-3D2
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human PSA (glandular kallikrein 3)
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
25,116 Da
OMIM
176820
3D Structure
ModBase 3D Structure for P07288
Buffer
phosphate buffered saline, pH 7.2
Immunogen
cDNA
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-KLK3 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry(IHC)
Application Notes for anti-KLK3 antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-KLK3 antibody

Fig.1: FACS analysis of BOSC23 cells using LT-3D2 Cat.# GM-0902. BOSC23 cells were transiently transfected with an expression vector encoding either PSA (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LT-3D2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSA transfected cells.
Mouse Anti-KLK3 Monoclonal Antibody – Cat# MBS468001

Testing Data #2 of anti-KLK3 antibody

Fig.2: SDS-PAGE analysis of purified LT-3D2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Mouse Anti-KLK3 Monoclonal Antibody, Testing Data #2 – Cat# MBS468001

Product References and Citations for anti-KLK3 antibody

Steven P Balk, Yoo-Joung Ko, Glenn J Bubley (2003). Biology of Prostate-Specific Antigen. Journal of Clinical Oncology 28 (2): 383-91.
Lilja H (2003). Biology of Prostate-Specific Antigen. Urology 62 (5 Suppl 1): 27-33. PMID 14607215.
Oesterling JE (1991): Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate. J Urol 145:907-923

Other Notes

Small volumes of anti-KLK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLK3 purchase

MBS468001 is an antibody targeting KLK3. Also known as anti-human Prostate-Specific Antigen (PSA) monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLK3. It may not necessarily be applicable to this product.

NCBI GI #
71834853
NCBI GeneID
354
NCBI Accession #
NP_001025218.1
UniProt Primary Accession #
P07288
UniProt Secondary Accession #
Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3
UniProt Related Accession #
P07288
GenBank Nucleotide #
NM_001030047.1
NCBI Official Full Name
prostate-specific antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
kallikrein-related peptidase 3
NCBI Official Symbol
KLK3
NCBI Official Synonym Symbols
APS; PSA; hK3; KLK2A1
UniProt Synonym Protein Names
Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
Protein Family
Glandular kallikrein
UniProt Gene Name
KLK3
UniProt Synonym Gene Names
APS; PSA; Seminin
UniProt Entry Name
KLK3_HUMAN
NCBI Protein Information
prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen

NCBI Summary for KLK3

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for KLK3

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.77; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis
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Research Articles on KLK3

1. High serum PSA levels > 100 ng/mL cis not be a prognostic factor in prostate cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLK3 antibody

 Reference Product  PubMed Publications
 TP53 antibody  >2 publications with KLK3 and TP53
 ERBB2 antibody  >2 publications with KLK3 and ERBB2
 IL6 antibody  >1 publications with KLK3 and IL6

Pathways associated with anti-KLK3 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Regulation Of Androgen Receptor Activity Pathway antibodies  Regulation Of Androgen Receptor Activity Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram

Diseases associated with anti-KLK3 antibody

 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >73 publications with KLK3 and Prostatic Neoplasms
 Adenocarcinoma Antibodies  >14 publications with KLK3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >14 publications with KLK3 and Breast Neoplasms
 Disease Progression Antibodies  >10 publications with KLK3 and Disease Progression
 Hyperplasia Antibodies  >9 publications with KLK3 and Hyperplasia
 Neoplasms, Experimental Antibodies  >6 publications with KLK3 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >6 publications with KLK3 and Kidney Diseases
 Neoplasm Metastasis Antibodies  >6 publications with KLK3 and Neoplasm Metastasis
 Ovarian Neoplasms Antibodies  >6 publications with KLK3 and Ovarian Neoplasms
 Hypertension Antibodies  >4 publications with KLK3 and Hypertension
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