Fig.1: FACS analysis of BOSC23 cells using GM-1E6 Cat.# MBS468007. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.
Fig.2: Spectral Confocal Microscopy of CHO cells using GM-1E7 Cat.# MBS468007. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of GM-1E7 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Fig 3: Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM human IL-4 (open circles) plus the indicated concentrations of purified GM- 1E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4- activity on TF-1 cells.
SDS-PAGE analysis of GM-1E7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified GM-1E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified GM-1E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
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