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Granzyme B (GZMB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468009

Background: Granzyme B (GrB) is a 27 kDa caspase-like serine protease that is released by activated cytotoxic T cells and natural killer cells to kill virus-infected and tumor cells (1). The enzymatic activity of granzyme B is considered essential to its… More details

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Product Overview

Product Name
Granzyme B (GZMB), Monoclonal Antibody
Full Product Name
anti-human Granzyme B monoclonal antibody
Product Gene Name
anti-GZMB antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GM-4C1
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human Granzyme B (GrB, granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
27,716 Da
OMIM
123910
3D Structure
ModBase 3D Structure for P10144
Buffer
phosphate buffered saline, pH 7.2
Immunogen
cDNA
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-GZMB antibody
Flow Cytometry (FC/FACS), CELISA, ELISA (EIA)
Application Notes for anti-GZMB antibody
Immunofluorescence: genetic immunisation with cDNA encoding human Granzyme B
ELISA: 1:200 - 1:400
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-GZMB antibody

Fig.1: FACS analysis of BOSC23 cells using GM-4C1 Cat.# GM-0201. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme B (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-4C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme B transfected cells.
Mouse Anti-GZMB Monoclonal Antibody – Cat# MBS468009

Testing Data #2 of anti-GZMB antibody

Fig.2: Intracellular detection of granzyme B in human PBMC by FACS analysis using GM-4C1. PBMC were cultivated in the presence of phorbolester and ionomycin subsequently fixed and permeabilised. Binding of GM-4C1 was detected with a FITC-conjugated secondary antibody.
Mouse Anti-GZMB Monoclonal Antibody, Testing Data #2 – Cat# MBS468009

Testing Data #3 of anti-GZMB antibody

Fig3: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an anti-tag monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, purified GM-4C1 was tested on all transfectants. A positive signal was obtained only with Granzyme B transfected cells (red curves).
Mouse Anti-GZMB Monoclonal Antibody, Testing Data #3 – Cat# MBS468009

Product References and Citations for anti-GZMB antibody

Trapani JA (2001). Granzymes a family of lymphocyte granule serine proteases. Genome Biol 2(12):REVIEWS3014.
Trapani JA, Sutton VR (2003). Granzyme B: pro-apoptotic, antiviral and antitumor functions. Curr Opin Immunol 15(5):533-43.
Graubert TA, DiPersio JF, Russell JH and Ley TJ (1997). Perforin/granzyme-dependent and Independent Mechanisms are Both Important for the Development of Graft-versus-host Disease After Murine Bone Marrow Transplantation. J Clin Invest 100(4): 904-911

Other Notes

Small volumes of anti-GZMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMB purchase

MBS468009 is an antibody targeting GZMB. Also known as anti-human Granzyme B monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

NCBI GI #
221625528
NCBI GeneID
3002
NCBI Accession #
NP_004122.2
UniProt Primary Accession #
P10144
UniProt Secondary Accession #
Q8N1D2; Q9UCC1
UniProt Related Accession #
P10144
GenBank Nucleotide #
NM_004131.4
NCBI Official Full Name
granzyme B
NCBI Official Synonym Full Names
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
NCBI Official Symbol
GZMB
NCBI Official Synonym Symbols
HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
UniProt Synonym Protein Names
C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
Protein Family
Granzyme
UniProt Gene Name
GZMB
UniProt Synonym Gene Names
CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
UniProt Entry Name
GRAB_HUMAN
NCBI Protein Information
granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2

NCBI Summary for GZMB

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
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UniProt Comments for GZMB

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.79; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; immune response; protein processing; proteolysis
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Research Articles on GZMB

1. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GZMB antibody

 Reference Product  PubMed Publications
 PRF1 antibody  >1245 publications with GZMB and PRF1
 CASP3 antibody  >141 publications with GZMB and CASP3
 BID antibody  >58 publications with GZMB and BID
 SERPINB9 antibody  >57 publications with GZMB and SERPINB9
 IL2RA antibody  >48 publications with GZMB and IL2RA
 CASP7 antibody  >19 publications with GZMB and CASP7
 IL2RB antibody  >14 publications with GZMB and IL2RB
 IGF2R antibody  >13 publications with GZMB and IGF2R
 IL2 antibody  >6 publications with GZMB and IL2

Pathways associated with anti-GZMB antibody

 Products by Pathway  Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram

Diseases associated with anti-GZMB antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >304 publications with GZMB and Necrosis
 Inflammation Antibodies  >194 publications with GZMB and Inflammation
 Disease Models, Animal Antibodies  >113 publications with GZMB and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >97 publications with GZMB and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >95 publications with GZMB and Nervous System Diseases
 Liver Diseases Antibodies  >74 publications with GZMB and Liver Diseases
 Hepatitis Antibodies  >54 publications with GZMB and Hepatitis
 Brain Diseases Antibodies  >35 publications with GZMB and Brain Diseases
 Breast Neoplasms Antibodies  >33 publications with GZMB and Breast Neoplasms
 Kidney Diseases Antibodies  >27 publications with GZMB and Kidney Diseases
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