Mouse Anti-CEACAM5 Monoclonal Antibody – Cat# MBS468019

Fig2: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6 or 8. Recognition of CEACAM3 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa cells. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM8: 80H3; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/5/1 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).

CEACAM5 Antibody – Mouse Monoclonal

Name: CEACAM5 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS468019
Price: 615 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468019

Background: CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 180,000-200,000 which is most strongly… More details

$615 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CEACAM5, Monoclonal Antibody

Full Product Name

anti-human CEACAM5 monoclonal antibody

Product Gene Name

anti-CEACAM5 antibody

Product Synonym Names

CD66e; CEA

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

26/5/1

Host

Mouse

Species Reactivity

Human

Form/Format

Purified

Purity/Purification

Protein G

Specificity

anti-human CEACAM5 (CEA; CD66e)

Preparation and Storage

Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)

Molecular Weight

76,724 Da

OMIM

114890

3D Structure

ModBase 3D Structure for P06731

Buffer

phosphate buffered saline, pH 7.2

Immunogen

extracted protein of CEACAM5

Selection

based on recognition of the complete native protein expressed on transfected mammalian cells

Applications Tested/Suitable for anti-CEACAM5 antibody

Flow Cytometry (FC/FACS), CELISA, ELISA (EIA), Western Blot (WB), Immunofluorescence(IF), Immunohistology on cryosections

Application Notes for anti-CEACAM5 antibody

Immunohistology: 1-2 ug/10^6 cells (on cryosections)
ELISA: 1:200 - 1:400
Western Blot: 4ug/ml
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Product References and Citations for anti-CEACAM5 antibody

Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
Grunert F, Stocks SC, Nagel G., Zimmermann W, Thompson JA, Jantscheff P and Kromer B. (1996). CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. In Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto et al., eds.), Garland Publishing Inc., New York and London, pp. 1012-1025.

Other Notes

Small volumes of anti-CEACAM5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CEACAM5 purchase

MBS468019 is an antibody targeting CEACAM5. Also known as anti-human CEACAM5 monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CEACAM5. It may not necessarily be applicable to this product.

NCBI GI #

612407802

NCBI GeneID

1048

NCBI Accession #

NP_001278413.1

UniProt Primary Accession #

P06731

UniProt Secondary Accession #

H9KVA7

UniProt Related Accession #

P06731

GenBank Nucleotide #

NM_001291484.1

NCBI Official Full Name

carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein

NCBI Official Synonym Full Names

carcinoembryonic antigen-related cell adhesion molecule 5

NCBI Official Symbol

CEACAM5

NCBI Official Synonym Symbols

CEA; CD66e

UniProt Synonym Protein Names

Carcinoembryonic antigen; CEA; Meconium antigen 100; CD_antigen: CD66e

Protein Family

Carcinoembryonic antigen-related cell adhesion molecule

UniProt Gene Name

CEACAM5

UniProt Synonym Gene Names

CEA; CEA

UniProt Entry Name

CEAM5_HUMAN

NCBI Protein Information

carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100

NCBI Summary for CEACAM5

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]

UniProt Comments for CEACAM5

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Membrane protein, GPI anchor; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis

Research Articles on CEACAM5

1. Overall survival of lung cancer patients was significantly longer in those with normal pre-treatment serum CEA levels than in those with abnormal pre-treatment serum CEA levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CEACAM5 antibody

Reference Product	PubMed Publications
CEACAM6 antibody	>112 publications with CEACAM5 and CEACAM6
HNRNPM antibody	>3 publications with CEACAM5 and HNRNPM
RAB11A antibody	>1 publications with CEACAM5 and RAB11A
VPS33B antibody	>1 publications with CEACAM5 and VPS33B
VIPAR antibody	>1 publications with CEACAM5 and VIPAR

Diseases associated with anti-CEACAM5 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>8317 publications with CEACAM5 and Carcinoma
Adenocarcinoma Antibodies	>4751 publications with CEACAM5 and Adenocarcinoma
Neoplasm Metastasis Antibodies	>2581 publications with CEACAM5 and Neoplasm Metastasis
Liver Neoplasms Antibodies	>2548 publications with CEACAM5 and Liver Neoplasms
Lung Neoplasms Antibodies	>2175 publications with CEACAM5 and Lung Neoplasms
Liver Diseases Antibodies	>1933 publications with CEACAM5 and Liver Diseases
Breast Neoplasms Antibodies	>1677 publications with CEACAM5 and Breast Neoplasms
Carcinoma, Squamous Cell Antibodies	>1057 publications with CEACAM5 and Carcinoma, Squamous Cell
Ovarian Neoplasms Antibodies	>830 publications with CEACAM5 and Ovarian Neoplasms
Neoplasms, Experimental Antibodies	>668 publications with CEACAM5 and Neoplasms, Experimental