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CCR4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468021

Background: CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane-bound molecules composed of 7- transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds the chemokines… More details

$615 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CCR4, Monoclonal Antibody
Full Product Name
anti-human CCR4 monoclonal antibody
Product Gene Name
anti-CCR4 antibody
Product Synonym Names
CC chemokine receptor 4
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KH-4F5
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human CCR4
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
41,403 Da
OMIM
604836
3D Structure
ModBase 3D Structure for P51679
Buffer
phosphate buffered saline, pH 7.2
Immunogen
peptide
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-CCR4 antibody
Flow Cytometry (FC/FACS), CELISA
Application Notes for anti-CCR4 antibody
Immunofluorescence: 1 ug/10^6 cells
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-CCR4 antibody

Fig.1: FACS analysis of BOSC23 cells using KH- 4F5 Cat.# GM-0602. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.
Mouse Anti-CCR4 Monoclonal Antibody – Cat# MBS468021

Testing Data #2 of anti-CCR4 antibody

Fig.2: Spectral Confocal Microscopy of CHO cells using KH-4F5 Cat.# GM-0602. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Mouse Anti-CCR4 Monoclonal Antibody, Testing Data #2 – Cat# MBS468021

Testing Data #3 of anti-CCR4 antibody

SDS-PAGE analysis of KH-4F5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.
Mouse Anti-CCR4 Monoclonal Antibody, Testing Data #3 – Cat# MBS468021

Product References and Citations for anti-CCR4 antibody

Andrew DP et al. (2001). C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential. J Immunol 166(1): 103-11
Murdoch C and Finn A (2000). Chemokine receptors and their role in inflammation and infectious diseases. Blood 95 (10): 3032-3043
Imai T et al. (1997). The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem 6;272(23):15036-42
Imai T et al. (1998). Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem 16;273(3):1764-8
Purandare AV and Somerville JE (2006). Antagonists of CCR4 as immunomodulatory agents. Curr Top Med Chem 6(13):1335-44

Other Notes

Small volumes of anti-CCR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCR4 purchase

MBS468021 is an antibody targeting CCR4. Also known as anti-human CCR4 monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CCR4. It may not necessarily be applicable to this product.

NCBI GI #
5031627
NCBI GeneID
1233
NCBI Accession #
NP_005499.1
UniProt Primary Accession #
P51679
UniProt Secondary Accession #
Q9ULY6; Q9ULY7
UniProt Related Accession #
P51679
GenBank Nucleotide #
NM_005508.4
NCBI Official Full Name
C-C chemokine receptor type 4
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 4
NCBI Official Symbol
CCR4
NCBI Official Synonym Symbols
CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
UniProt Synonym Protein Names
K5-5; CD_antigen: CD194
Protein Family
C-C chemokine receptor
UniProt Gene Name
CCR4
UniProt Synonym Gene Names
CMKBR4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4
UniProt Entry Name
CCR4_HUMAN
NCBI Protein Information
C-C chemokine receptor type 4; CCR-4; C-C CKR-4; chemokine (C-C) receptor 4

NCBI Summary for CCR4

The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]
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UniProt Comments for CCR4

CCR4: High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol- calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: cell soma; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity

Biological Process: response to antibiotic; G-protein coupled receptor protein signaling pathway; response to radiation; elevation of cytosolic calcium ion concentration; positive regulation of positive chemotaxis; response to bacterium; neuron migration; immune response; inflammatory response; chemotaxis; tolerance induction
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Research Articles on CCR4

1. Data show that CCR4 exists in at least two distinct conformations, which are differentially activated by ligand.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCR4 antibody

 Reference Product  PubMed Publications
 CCL17 antibody  >242 publications with CCR4 and CCL17
 CCL22 antibody  >216 publications with CCR4 and CCL22
 CCL5 antibody  >87 publications with CCR4 and CCL5
 CCL3 antibody  >45 publications with CCR4 and CCL3
 CXCL12 antibody  >41 publications with CCR4 and CXCL12
 CCL27 antibody  >20 publications with CCR4 and CCL27
 CCL13 antibody  >10 publications with CCR4 and CCL13
 CCL26 antibody  >7 publications with CCR4 and CCL26
 ADRBK1 antibody  >2 publications with CCR4 and ADRBK1
 ADRBK2 antibody  >1 publications with CCR4 and ADRBK2

Pathways associated with anti-CCR4 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram

Diseases associated with anti-CCR4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >269 publications with CCR4 and Inflammation
 Lung Diseases Antibodies  >133 publications with CCR4 and Lung Diseases
 Necrosis Antibodies  >82 publications with CCR4 and Necrosis
 Carcinoma Antibodies  >37 publications with CCR4 and Carcinoma
 Drug Hypersensitivity Antibodies  >35 publications with CCR4 and Drug Hypersensitivity
 Fibrosis Antibodies  >29 publications with CCR4 and Fibrosis
 Neoplasms, Experimental Antibodies  >20 publications with CCR4 and Neoplasms, Experimental
 Heart Diseases Antibodies  >19 publications with CCR4 and Heart Diseases
 Liver Diseases Antibodies  >18 publications with CCR4 and Liver Diseases
 Lung Neoplasms Antibodies  >16 publications with CCR4 and Lung Neoplasms
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