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CRISP3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468024

Background: Cysteine-rich secretory protein 3 (CRISP3) belongs to the cysteine-rich secretory protein family. CRISPs are characterized by a cysteine-rich domain at the COOH terminal that form 8 intramolecular disulfide bonds (1). Mammalian members of the… More details

$615 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Crisp3, Monoclonal Antibody
Full Product Name
anti-human Cysteine-rich secretory protein 3 (Crisp3) monoclonal antibody
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LV-2A2
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human cysteine-rich secretory protein 3 (Crisp3, SGP28)
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
30,116 Da
3D Structure
ModBase 3D Structure for P54108
Buffer
phosphate buffered saline, pH 7.2
Immunogen
cDNA
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-CRISP3 antibody
Flow Cytometry (FC/FACS), CELISA
Application Notes for anti-CRISP3 antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-CRISP3 antibody

Fig.1: FACS analysis of BOSC23 cells using LV-2A2 Cat.# GM-0904. BOSC23 cells were transiently transfected with an expression vector encoding either Crisp3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LV-2A2 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with Crisp3 transfected cells.
Mouse Anti-CRISP3 Monoclonal Antibody – Cat# MBS468024

Testing Data #2 of anti-CRISP3 antibody

Fig.2: SDS-PAGE analysis of purified LV-2A2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LV-2A2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Mouse Anti-CRISP3 Monoclonal Antibody, Testing Data #2 – Cat# MBS468024

Product References and Citations for anti-CRISP3 antibody

Krätzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD (1996). The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 Eur J Biochem 15;236(3):827-36
Udby L, Bjartell A, Malm J, Egesten A, Lundwall A, Cowland JB, Borregaard N, Kjeldsen L (2005). Characterization and Localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the Human Male Reproductive Tract. J Androl ;26(3):333-42
Kosari F, Asmann YW, Cheville JC and Vasmatzis G (2002). Cysteine-rich Secretory Protein- 3: A Potential Biomarker for Prostate Cancer. Cancer Epidemiology Biomarkers & Prevention 11;1419-1426

Other Notes

Small volumes of anti-CRISP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRISP3 purchase

MBS468024 is an antibody targeting CRISP3. Also known as anti-human Cysteine-rich secretory protein 3 (Crisp3) monoclonal antibody . Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CRISP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cysteine-rich secretory protein 3 isoform 2
NCBI Official Synonym Full Names
cysteine-rich secretory protein 3
NCBI Official Symbol
CRISP3
NCBI Official Synonym Symbols
Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
UniProt Synonym Protein Names
Specific granule protein of 28 kDa; SGP28
UniProt Gene Name
CRISP3
UniProt Synonym Gene Names
CRISP-3; SGP28
UniProt Entry Name
CRIS3_HUMAN
NCBI Protein Information
cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; specific granule protein (28 kDa); specific granule protein of 28 kDa

UniProt Comments for CRISP3

CRISP3: Belongs to the CRISP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; specific granule; extracellular region

Biological Process: innate immune response; defense response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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