• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LGR4 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS468030
SPECIAL PRICING: 1 unit is in stock at 40% OFF web price. Please contact customer service for more information

Background:LGR4 (Leucine-rich repeat- containing G protein-coupled receptor 4) belongs to the superfamily of G protein-coupled receptors (GPCRs). It is a 951 amino acid multi-pass membrane protein and has multiple N-terminal leucine-rich repeats, which are… More details

$615 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LGR4, Monoclonal Antibody
Full Product Name
Anti-LGR4 (GPR48) monoclonal antibody
Product Gene Name
anti-LGR4 antibody
Product Synonym Names
GPR48
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
BBX-3A8
Host
Rat
Species Reactivity
Human, Mouse
Purity/Purification
Protein G
Specificity
anti-human LGR4, cross reactivity with murine LGR4
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
OMIM
gene 615311
3D Structure
ModBase 3D Structure for Q9BXB1
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunization with cDNA encoding human LGR4
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-LGR4 antibody
Flow Cytometry (FC/FACS), CELISA
Application Notes for anti-LGR4 antibody
Flow Cytometry:1.2 ug/106cells
CELISA:1:200 - 1:400
For each application a titration should be performed to determined the optimal concentration.

Testing Data of anti-LGR4 antibody

Fig.1: FACS analysis of BOSC23 cells using BBX-3A8 Cat.# MBS468030. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained with LGR4- hum and LGR4-mur transfected cells.
Rat Anti-LGR4 Monoclonal Antibody – Cat# MBS468030

SDS-PAGE of anti-LGR4 antibody

SDS-PAGE analysis purifed BBX-3A8 monoclonal antibody.
Lane 1: molecular weight marker
Lane 2: 2ug of purified BBX-3A8 antibody.
Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Rat Anti-LGR4 Monoclonal Antibody, SDS-PAGE – Cat# MBS468030

Product References and Citations for anti-LGR4 antibody

Hsu SY, Liang SG, Hsueh AJ (1998). Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G proteincoupled, seven-transmembrane region. Mol Endocrinol. 12(12):1830-45
Loh ED, Broussard SR, Liu Q, Copeland NG, Gilbert DJ, Jenkins NA, Kolakowski, LF Jr.(2000). Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet Cell Genet. 89(1-2):2-5
Loh ED, Broussard SR, Kolakowski LF (2001). Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem Biophys Res Commun. 282(3):757-64

Other Notes

Small volumes of anti-LGR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGR4 purchase

MBS468030 is an antibody targeting LGR4. Also known as Anti-LGR4 (GPR48) monoclonal antibody. Reactive in Human, Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for LGR4. It may not necessarily be applicable to this product.

NCBI GI #
157694513
NCBI GeneID
55366
NCBI Accession #
NP_060960.2
UniProt Primary Accession #
Q9BXB1
UniProt Secondary Accession #
Q8N537; Q9NYD1; A6NCH3; G5E9B3
UniProt Related Accession #
Q9BXB1
GenBank Nucleotide #
NM_018490.2
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 4
NCBI Official Synonym Full Names
leucine-rich repeat containing G protein-coupled receptor 4
NCBI Official Symbol
LGR4
NCBI Official Synonym Symbols
GPR48; BNMD17
UniProt Synonym Protein Names
G-protein coupled receptor 48
Protein Family
Leucine-rich repeat-containing G-protein coupled receptor
UniProt Gene Name
LGR4
UniProt Synonym Gene Names
GPR48
UniProt Entry Name
LGR4_HUMAN
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 4; G protein-coupled receptor 48

NCBI Summary for LGR4

G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).[supplied by OMIM, Aug 2008]

UniProt Comments for LGR4

LGR4: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Plays an essential role during homeostasis of the adult mouse intestine.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11p14-p13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: osteoblast differentiation; G-protein coupled receptor protein signaling pathway; epithelial cell proliferation; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; negative regulation of toll-like receptor signaling pathway; male genitalia development; innate immune response; spermatogenesis; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; bone remodeling; bone mineralization

Disease: Bone Mineral Density Quantitative Trait Locus 17
Show more

Research Articles on LGR4

1. our results suggest a previously unknown Stat3-LGR4 molecular network, which may control osteosarcoma development and progression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LGR4 antibody

 Reference Product  PubMed Publications
 ZNRF3 antibody  >8 publications with LGR4 and ZNRF3
 ATF4 antibody  >2 publications with LGR4 and ATF4
 PITX2 antibody  >2 publications with LGR4 and PITX2
 ESR1 antibody  >1 publications with LGR4 and ESR1

Pathways associated with anti-LGR4 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
 XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies  XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
 Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies  Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
 Regulation Of FZD By Ubiquitination Pathway antibodies  Regulation Of FZD By Ubiquitination Pathway Diagram

Diseases associated with anti-LGR4 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >4 publications with LGR4 and Kidney Diseases
 Infertility, Female Antibodies  >3 publications with LGR4 and Infertility, Female
 Inflammation Antibodies  >3 publications with LGR4 and Inflammation
 Neoplasms, Experimental Antibodies  >2 publications with LGR4 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >2 publications with LGR4 and Disease Models, Animal
 Liver Diseases Antibodies  >1 publications with LGR4 and Liver Diseases
 Abnormalities, Drug-Induced Antibodies  >1 publications with LGR4 and Abnormalities, Drug-Induced
 Necrosis Antibodies  >1 publications with LGR4 and Necrosis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000