Fig.l: FC analysis of BOSC23 cells using MUS Cat # MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAMS (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAMS transfected cells.
Fig.2: FACS analysis of BOSC23 cells using MUS Cat.# MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM6 transfected cells.
Fig. 3: Specificity testing of MUS. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified MUS was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM5 and CEACAM6 expressing cells (red curves).
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