Fig.1: FACS analysis of BOSC23 cells using SU-9D5 Cat.# GM-0518. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). Binding of SU-9D5 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM16 transfected cells.
Fig.2: Immunohistological staining using SU-9D5 Cat.# GM-0518 on paraffin sections of cochlea tissue of an adult transgenic mouse with a human CEACAM16 gene (Ceacam16+/+ mouse cochlea). Binding of biotinylated SU-9D5 was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole. Constanze Schäuble, Tumor Immunology Laboratory, LMU, Munich
Fig.3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified SU-9D5 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM16 transfected cells (red curve).
SDS-PAGE analysis of SU-9D5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified SU-9D5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified SU-9D5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.
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