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Granzyme K (GZMK) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468035

Background: Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme… More details

$615 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Granzyme K (GZMK), Monoclonal Antibody
Full Product Name
anti-human Granzyme K monoclonal antibody
Product Gene Name
anti-GZMK antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GM-6C3
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human Granzyme K (GrK; granzyme-3; NK-tryptase-2)
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
28,882 Da
OMIM
600784
3D Structure
ModBase 3D Structure for P49863
Buffer
phosphate buffered saline, pH 7.2
Immunogen
cDNA
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-GZMK antibody
ELISA (EIA) pair with GM26E7 (catcher), Flow Cytometry (FC/FACS), CELISA, ELISA (EIA)
Application Notes for anti-GZMK antibody
Immunofluorescence: genetic immunisation with cDNA encoding human Granzyme K
ELISA: 1:200 - 1:400
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-GZMK antibody

Fig.1: FACS analysis of BOSC23 cells using GM-6C3 Cat.# GM- 0205. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme K (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-6C3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme K transfected cells.
Mouse Anti-GZMK Monoclonal Antibody – Cat# MBS468035

Testing Data #2 of anti-GZMK antibody

Fig2: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an antimyc monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, GM-6C3 hybridoma supernatant was tested on all transfectants. A positive signal was obtained only with Granzyme K transfected cells (red curves).
Mouse Anti-GZMK Monoclonal Antibody, Testing Data #2 – Cat# MBS468035

Product References and Citations for anti-GZMK antibody

Bade B, Lohrmann J, ten Brinke A, Wolbink AM, Wolbink GJ, ten Berge IJM, Virchow JC Jr, Luttmann W and CE Hack (2005). Detection of soluble human granzyme K in vitro and in vivo. Eur. J. Immunol. (10): 2940-2948.
Bade B, Boettcher HE, Lohrmann J, Hink-Schauer C, Bratke K, Jenne DE, Virchow JC Jr and W. Luttmann (2005). Differential expression of the granzymes A, K and M and perforin in human peripheral blood lymphocytes. Int Immunol. (11): 1419-1428.

Other Notes

Small volumes of anti-GZMK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMK purchase

MBS468035 is an antibody targeting GZMK. Also known as anti-human Granzyme K monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMK. It may not necessarily be applicable to this product.

NCBI GI #
4504235
NCBI GeneID
3003
NCBI Accession #
NP_002095.1
UniProt Primary Accession #
P49863
UniProt Secondary Accession #
B2R563
UniProt Related Accession #
P49863
GenBank Nucleotide #
NM_002104.2
NCBI Official Full Name
granzyme K
NCBI Official Synonym Full Names
granzyme K (granzyme 3; tryptase II)
NCBI Official Symbol
GZMK
NCBI Official Synonym Symbols
TRYP2
UniProt Synonym Protein Names
Fragmentin-3; Granzyme-3; NK-tryptase-2; NK-Tryp-2
Protein Family
Granzyme
UniProt Gene Name
GZMK
UniProt Synonym Gene Names
TRYP2; NK-Tryp-2
UniProt Entry Name
GRAK_HUMAN
NCBI Protein Information
granzyme K; NK-Tryp-2; granzyme 3; granzyme-3; tryptase II; fragmentin-3; NK-tryptase-2; granzyme K (serine protease, granzyme 3; tryptase II)

NCBI Summary for GZMK

This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]
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UniProt Comments for GZMK

GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis
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Research Articles on GZMK

1. Extracellular GrK is an unexpected direct modulator of lipopolysaccharide-TLR4 signaling during the antimicrobial innate immune response.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GZMK antibody

 Reference Product  PubMed Publications
 GZMB antibody  >35 publications with GZMK and GZMB
 GZMA antibody  >12 publications with GZMK and GZMA
 PRF1 antibody  >8 publications with GZMK and PRF1
 CCR5 antibody  >1 publications with GZMK and CCR5
 GZMH antibody  >1 publications with GZMK and GZMH
 CD2 antibody  >1 publications with GZMK and CD2

Diseases associated with anti-GZMK antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4 publications with GZMK and Inflammation
 Chromosome Aberrations Antibodies  >2 publications with GZMK and Chromosome Aberrations
 Heart Defects, Congenital Antibodies  >1 publications with GZMK and Heart Defects, Congenital
 Cardiovascular Diseases Antibodies  >1 publications with GZMK and Cardiovascular Diseases
 Lung Diseases Antibodies  >1 publications with GZMK and Lung Diseases
 Pneumonia Antibodies  >1 publications with GZMK and Pneumonia
 Depressive Disorder Antibodies  >1 publications with GZMK and Depressive Disorder
 Necrosis Antibodies  >1 publications with GZMK and Necrosis
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