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ICOSLG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468040

Background: Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS)(1). ICOS belongs to the CD28/CD152 receptor family that regulates… More details

$635 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ICOSL (ICOSLG), Monoclonal Antibody
Full Product Name
anti-human Inducible co-stimulator ligand (ICOSL) monoclonal antibody
Product Gene Name
anti-ICOSLG antibody
Product Synonym Names
ICOS ligand
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GM-13C1
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human Inducible co-stimulator ligand (ICOSL)
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
20,120 Da
OMIM
605717
3D Structure
ModBase 3D Structure for O75144
Immunogen
cDNA
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Buffer
phosphate buffered saline, pH 7.2
Applications Tested/Suitable for anti-ICOSLG antibody
Flow Cytometry (FC/FACS)and CELISA
Application Notes for anti-ICOSLG antibody
Immunofluorescence: genetic immunisation with cDNA encoding human ICOSL (extracellular domain)
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-ICOSLG antibody

Fig.1: FACS analysis of BOSC23 cells using GM-13C1 Cat.# GM-0301. BOSC23 cells were transiently transfected with an expression vector encoding either ICOSL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-13C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ICOSL transfected cells.
Mouse Anti-ICOSLG Monoclonal Antibody – Cat# MBS468040

Testing Data #2 of anti-ICOSLG antibody

SDS-PAGE analysis of GM-13C1 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-13C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-13C1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.
Mouse Anti-ICOSLG Monoclonal Antibody, Testing Data #2 – Cat# MBS468040

Product References and Citations for anti-ICOSLG antibody

Richter G, Hayden-Ledbetter M, Irgang M, Ledbetter JA, Westermann J, Korner I, Daemen K, Clark EA, Aicher A and Pezzutto A (2001). Tumor Necrosis Factor-? Regulates the Expression of Inducible Costimulator Receptor Ligand on CD34+ Progenitor Cells during Differentiation into Antigen Presenting Cells. J Biol Chem 276: 45686-45693.
Richter G and Burdach S (2004). ICOS: a new costimulatory ligand/receptor pair and its role in T-cell activation. Onkologie 27(1): 91-5
Aicher A, Hayden-Ledbetter M, Brady WA, Pezzutto A, Richer G, Magaletti D, Buckwalter S, Ledbetter JA and Clark EA (2000). Characterization of human inducible costimulator ligand expression and function. J Immunol 1;164(9):4689-96

Other Notes

Small volumes of anti-ICOSLG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICOSLG purchase

MBS468040 is an antibody targeting ICOSLG. Also known as anti-human Inducible co-stimulator ligand (ICOSL) monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ICOSLG. It may not necessarily be applicable to this product.

NCBI GI #
545688560
NCBI GeneID
23308
NCBI Accession #
NP_001269979.1
UniProt Primary Accession #
O75144
UniProt Secondary Accession #
Q9HD18; Q9NRQ1; A8MUZ1
UniProt Related Accession #
O75144
GenBank Nucleotide #
NM_001283050.1
NCBI Official Full Name
ICOS ligand isoform b
NCBI Official Synonym Full Names
inducible T-cell co-stimulator ligand
NCBI Official Symbol
ICOSLG
NCBI Official Synonym Symbols
B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
UniProt Synonym Protein Names
B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD_antigen: CD275
Protein Family
ICOS ligand
UniProt Gene Name
ICOSLG
UniProt Synonym Gene Names
B7H2; B7RP1; ICOSL; KIAA0653; B7-H2; B7RP-1
UniProt Entry Name
ICOSL_HUMAN
NCBI Protein Information
ICOS ligand; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; transmembrane protein B7-H2 ICOS ligand

UniProt Comments for ICOSLG

ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: receptor binding

Biological Process: hyperosmotic response; B cell activation; T cell activation; T cell costimulation; defense response; positive regulation of activated T cell proliferation; signal transduction
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Research Articles on ICOSLG

1. a critical role is described for the rs7282490 ICOSLG region polymorphism associated with immune-mediated diseases in amplifying pattern-recognition receptor initiated inflammatory signaling and cytokine secretion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ICOSLG antibody

 Reference Product  PubMed Publications
 ICOS antibody  >58 publications with ICOSLG and ICOS
 TNFSF18 antibody  >4 publications with ICOSLG and TNFSF18
 PTPRC antibody  >1 publications with ICOSLG and PTPRC

Pathways associated with anti-ICOSLG antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram

Diseases associated with anti-ICOSLG antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >26 publications with ICOSLG and Immune System Diseases
 Necrosis Antibodies  >21 publications with ICOSLG and Necrosis
 Autoimmune Diseases Antibodies  >15 publications with ICOSLG and Autoimmune Diseases
 Inflammation Antibodies  >14 publications with ICOSLG and Inflammation
 Asthma Antibodies  >7 publications with ICOSLG and Asthma
 Carcinoma Antibodies  >6 publications with ICOSLG and Carcinoma
 Lung Diseases Antibodies  >6 publications with ICOSLG and Lung Diseases
 Cardiovascular Diseases Antibodies  >4 publications with ICOSLG and Cardiovascular Diseases
 Colitis, Ulcerative Antibodies  >3 publications with ICOSLG and Colitis, Ulcerative
 Celiac Disease Antibodies  >3 publications with ICOSLG and Celiac Disease
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