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ACPP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS468044

Background: Human prostatic acid phosphatase (PAP) is a non-specific phosphomonoesterase, synthesised and secreted into seminal plasma under androgenic control. Human PAP is a 100 kDa glycoprotein containing two subunits of approximately 50 kDa each (1,2). It… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PAP (ACPP), Monoclonal Antibody
Full Product Name
anti-human Prostatic Acid Phosphatase (PAP) monoclonal antibody
Product Gene Name
anti-ACPP antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT-3D1
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
Purity/Purification
Protein G
Specificity
anti-human PAP (PAcP, ACPP)
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Molecular Weight
40,443 Da
OMIM
171790
3D Structure
ModBase 3D Structure for P15309
Buffer
phosphate buffered saline, pH 7.2
Immunogen
cDNA
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Applications Tested/Suitable for anti-ACPP antibody
Flow Cytometry (FC/FACS), CELISA
Application Notes for anti-ACPP antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-ACPP antibody

Fig.1: FACS analysis of BOSC23 cells using LT-3D1 Cat.# GM- 0907. BOSC23 cells were transiently transfected with an expression vector encoding either PAP (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LT-3D1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PAP transfected cells.
Mouse Anti-ACPP Monoclonal Antibody – Cat# MBS468044

Testing Data #2 of anti-ACPP antibody

Fig.2: SDS-PAGE analysis of purified LT-3D1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Mouse Anti-ACPP Monoclonal Antibody, Testing Data #2 – Cat# MBS468044

Product References and Citations for anti-ACPP antibody

Ostrowski WS, Kuciel R (1994). Human prostatic acid phosphatase: selected properties and practical applications. Clin Chim Acta 226(2):121-9
Bilhartz DL, Tindall DJ, Oesterling JE (1991). Prostate-specific antigen and prostatic acid phosphatase: biomolecular and physiologic characteristics. Urology 38(2):95-102
Veeramani S, Yuan TC, Chen SJ, Lin FF, Petersen JE, Shaheduzzaman S, Srivastava S, MacDonald RG, Lin MF (2005). Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer 12(4):805-22

Other Notes

Small volumes of anti-ACPP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACPP purchase

MBS468044 is an antibody targeting ACPP. Also known as anti-human Prostatic Acid Phosphatase (PAP) monoclonal antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACPP. It may not necessarily be applicable to this product.

NCBI GI #
6382064
NCBI GeneID
55
NCBI Accession #
NP_001090.2
UniProt Primary Accession #
P15309
UniProt Secondary Accession #
Q5FBY0; Q96KY0; Q96QK9; Q96QM0; D3DNC6
UniProt Related Accession #
P15309
GenBank Nucleotide #
NM_001099.4
NCBI Official Full Name
prostatic acid phosphatase isoform PAP
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
ACPP
NCBI Official Synonym Symbols
ACP3; 5'-NT; ACP-3
UniProt Synonym Protein Names
5'-nucleotidase (EC:3.1.3.5); 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPase
Protein Family
Acyl carrier protein
UniProt Gene Name
ACPP
UniProt Synonym Gene Names
PAP; 5'-NT; TMPase
UniProt Entry Name
PPAP_HUMAN
NCBI Protein Information
prostatic acid phosphatase; TMPase; 5'-nucleotidase; ecto-5'-nucleotidase; thiamine monophosphatase; prostatic acid phosphotase

NCBI Summary for ACPP

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
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UniProt Comments for ACPP

ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.5; EC 3.1.3.2; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: multivesicular body; extracellular space; Golgi cisterna; apical part of cell; lysosomal membrane; integral to membrane; plasma membrane; intracellular; vesicle membrane; nucleus; filopodium; secretory granule

Molecular Function: 5'-nucleotidase activity; identical protein binding; choline binding; acid phosphatase activity; protein homodimerization activity; phosphoric monoester hydrolase activity; thiamin phosphate phosphatase activity

Biological Process: thiamin metabolic process; dephosphorylation; positive regulation of adenosine receptor signaling pathway; nucleotide metabolic process; regulation of sensory perception of pain; adenosine metabolic process; purine base metabolic process
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Research Articles on ACPP

1. GCNT1 is over-expressed in prostate cancer and is associated with higher levels of core 2 O-sLe(x) in PSA, PAP and MUC1 proteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACPP antibody

 Reference Product  PubMed Publications
 KLK3 antibody  >1 publications with ACPP and KLK3
 ALPL antibody  >1 publications with ACPP and ALPL

Pathways associated with anti-ACPP antibody

 Products by Pathway  Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram

Diseases associated with anti-ACPP antibody

 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >8 publications with ACPP and Prostatic Neoplasms
 Inflammation Antibodies  >4 publications with ACPP and Inflammation
 Pain Antibodies  >4 publications with ACPP and Pain
 Breast Neoplasms Antibodies  >3 publications with ACPP and Breast Neoplasms
 Fibrosis Antibodies  >3 publications with ACPP and Fibrosis
 Necrosis Antibodies  >3 publications with ACPP and Necrosis
 Hyperplasia Antibodies  >3 publications with ACPP and Hyperplasia
 Neoplasm Metastasis Antibodies  >2 publications with ACPP and Neoplasm Metastasis
 Urinary Bladder Neoplasms Antibodies  >2 publications with ACPP and Urinary Bladder Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with ACPP and Cardiovascular Diseases
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