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CDC42 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS470007

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cdc42, Monoclonal Antibody
Full Product Name
Cdc42
Product Gene Name
anti-CDC42 antibody
Product Synonym Names
Cell Division Cycle 42; GTP-Binding Protein 25-KD; G25K
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M152
Host
Mouse
Species Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Specificity
The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.
Preparation and Storage
Store at –20°C. Stable for 1 year.
Chromosome Location
Chromosome: 1; NC_000001.10 (22379120..22419436). Location: 1p36.1
3D Structure
ModBase 3D Structure for Q5JYX0
Immunogen
Clone (M152) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
Applications Tested/Suitable for anti-CDC42 antibody
ELISA, Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-CDC42 antibody
ELISA: 1:2000
Western Blot: 1:500
Immunocytochemistry: 1:50

End user should determine optimal dilution for their particular applications and experiments. Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Immunocytochemistry (ICC) of anti-CDC42 antibody

Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (CM1521) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.
Mouse Anti-CDC42 Monoclonal Antibody – Cat# MBS470007

Western Blot (WB) of anti-CDC42 antibody

Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).
Mouse Anti-CDC42 Monoclonal Antibody, Western Blot (WB) – Cat# MBS470007

Product References and Citations for anti-CDC42 antibody

• Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977.
• Shinjo, K. et al. (1990) PNAS (USA) 87:9853.
• Erickson, J.W. & Cerione, R.A. (2001) Curr. Opin. Cell. Biol. 13(2):153.

Other Notes

Small volumes of anti-CDC42 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC42 purchase

MBS470007 is an antibody targeting CDC42. Reactive in Human, Rat, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC42. It may not necessarily be applicable to this product.

NCBI GI #
56202836
NCBI GeneID
998
NCBI Accession #
CAI19851.1
UniProt Primary Accession #
Q5JYX0
UniProt Related Accession #
P60953; Q5JYX0; Q9UJM0; Q9UJM1
GenBank Nucleotide #
AL031281.6
NCBI Official Full Name
cell division cycle 42 (GTP binding protein, 25kDa)
NCBI Official Synonym Full Names
cell division cycle 42 (GTP binding protein, 25kDa)
NCBI Official Symbol
CDC42
NCBI Official Synonym Symbols
G25K; CDC42Hs
UniProt Protein Name
Cell division cycle 42 (GTP binding protein, 25kDa)
Protein Family
Cdc42
UniProt Gene Name
CDC42
UniProt Entry Name
Q5JYX0_HUMAN
NCBI Protein Information
cell division control protein 42 homolog; OTTHUMP00000002834; OTTHUMP00000002926; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))

NCBI Summary for CDC42

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
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UniProt Comments for CDC42

Sequence similarities: Belongs to the small GTPase superfamily. Rho family. RuleBase RU003317

Research Articles on CDC42

1. These results demonstrate an important role for FGD1/Cdc42 signaling in human mesenchymal stem cells osteogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC42 antibody

 Reference Product  PubMed Publications
 PAK1 antibody  >270 publications with CDC42 and PAK1
 IQGAP1 antibody  >103 publications with CDC42 and IQGAP1
 RAC2 antibody  >100 publications with CDC42 and RAC2
 PAK2 antibody  >75 publications with CDC42 and PAK2
 VAV1 antibody  >55 publications with CDC42 and VAV1
 PAK4 antibody  >40 publications with CDC42 and PAK4
 TNK2 antibody  >36 publications with CDC42 and TNK2
 FGD1 antibody  >33 publications with CDC42 and FGD1
 CASP3 antibody  >32 publications with CDC42 and CASP3
 ACTR3 antibody  >31 publications with CDC42 and ACTR3

Pathways associated with anti-CDC42 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram

Diseases associated with anti-CDC42 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >567 publications with CDC42 and Neoplasms
 Nervous System Diseases Antibodies  >109 publications with CDC42 and Nervous System Diseases
 Inflammation Antibodies  >95 publications with CDC42 and Inflammation
 Neoplasm Metastasis Antibodies  >92 publications with CDC42 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >89 publications with CDC42 and Breast Neoplasms
 Necrosis Antibodies  >72 publications with CDC42 and Necrosis
 Adenocarcinoma Antibodies  >63 publications with CDC42 and Adenocarcinoma
 Disease Models, Animal Antibodies  >63 publications with CDC42 and Disease Models, Animal
 Gastrointestinal Diseases Antibodies  >61 publications with CDC42 and Gastrointestinal Diseases
 Cardiovascular Diseases Antibodies  >55 publications with CDC42 and Cardiovascular Diseases

Organs/Tissues associated with anti-CDC42 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >456 publications with CDC42 and Blood
 Brain Antibodies  >354 publications with CDC42 and Brain
 Nerve Antibodies  >269 publications with CDC42 and Nerve
 Vascular Antibodies  >234 publications with CDC42 and Vascular
 Muscle Antibodies  >185 publications with CDC42 and Muscle
 Kidney Antibodies  >173 publications with CDC42 and Kidney
 Embryonic Tissue Antibodies  >131 publications with CDC42 and Embryonic Tissue
 Lung Antibodies  >118 publications with CDC42 and Lung
 Bone Antibodies  >99 publications with CDC42 and Bone
 Liver Antibodies  >81 publications with CDC42 and Liver
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