3a/B (Tyr-279/Tyr-216) (GSK) Antibody – Mouse Monoclonal

Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (alpha and beta) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3beta plays a critical role in cell survival by phosphorylating nuclear factor-kappa-B (NF- kappa-B) p65 subunit, leading to NF-kappa-B transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3 alpha), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of beta-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3beta activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3beta (Serine 21 in GSK-3alpha). This phosphorylation may be involved in later phases of neuronal apoptosis.

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• Takahashi-Yanaga, F. et al. (2004) Biochem Biophys Res Commun. 316(2):411.
• Xinbo, L. et al. (2003) Mol Cancer Ther. 2:1215.
• Ding, Y. et al. (2008) J Neurosci. 28(45):11622. (WB: Mouse cortex, hippocampus)

Small volumes of anti-GSK-3a/b (Tyr-279/Tyr-216) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS470015 is an antibody targeting GSK-3a/b (Tyr-279/Tyr-216). Also known as GSK-3alpha/beta (Tyr-279/Tyr-216), phosphospecific. Reactive in Human, Rat, Mouse. Host: Mouse.