Rabbit Anti-iNOS Polyclonal Antibody – Cat# MBS472029

Inducible Nitric Oxide Synthase (iNOS) Antibody – Rabbit Polyclonal

Name: iNOS Antibody; Polyclonal; Rabbit; Human/Rat/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS472029
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS472029

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves… More details

$435 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Inducible Nitric Oxide Synthase (iNOS), Polyclonal Antibody

Full Product Name

Inducible Nitric Oxide Synthase

Product Gene Name

anti-iNOS antibody

Product Synonym Names

iNOS; NOS type II; Inducible nitric oxide synthase; Hepatocyte NOS

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Rat, Mouse

Form/Format

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Purity/Purification

Affinity Purified

Specificity

The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages treated with IFNg and LPS.

Preparation and Storage

Store at -20 degree C. Do not aliquot. Stable for 1 year.

Molecular Weight

130,628 Da

3D Structure

ModBase 3D Structure for Q06518

Immunogen

Amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.

Applications Tested/Suitable for anti-iNOS antibody

ELISA, Western Blot

Application Notes for anti-iNOS antibody

ELISA: 1:4000
Western Blot: 1:2000

Product References and Citations for anti-iNOS antibody

• Kleinert, H. et al. (2003) Biol Chem. 384(10-11):1343.
• Xie, Q.W. et al. (1992) Science 256:225.

Other Notes

Small volumes of anti-iNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for iNOS purchase

MBS472029 is an antibody targeting iNOS. Also known as Inducible Nitric Oxide Synthase. Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for iNOS. It may not necessarily be applicable to this product.

NCBI GI #

2935553

NCBI Accession #

AAC83553.1

UniProt Primary Accession #

Q06518

UniProt Secondary Accession #

O35765; O35766; O60591; O60604; P97774; Q63267; Q64005; Q64558

GenBank Nucleotide #

AF049656.1

NCBI Official Full Name

inducible nitric oxide synthase

NCBI Official Symbol

iNOS

UniProt Protein Name

Nitric oxide synthase, inducible

UniProt Synonym Protein Names

Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

UniProt Gene Name

Nos2

UniProt Entry Name

NOS2_RAT

UniProt Comments for iNOS

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

Catalytic activity: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.

Enzyme regulation: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity

Subunit structure: Homodimer. Binds SLC9A3R1

Tissue specificity: In normal kidney, expressed primarily in the medullary thick ascending limb, with minor amounts in the medullary collecting duct and vasa recta bundle.

Induction: By interferon gamma and lipopolysaccharides (LPS).

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

Caution: sequence Was originally (Ref.9) thought to originate from human but appears to be from rat.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-iNOS antibody

Reference Product	PubMed Publications
FLNA antibody	>12 publications with iNOS and FLNA

Pathways associated with anti-iNOS antibody

Products by Pathway	Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram

Diseases associated with anti-iNOS antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>1541 publications with iNOS and Inflammation
Cardiovascular Diseases Antibodies	>794 publications with iNOS and Cardiovascular Diseases
Drug Toxicity Antibodies	>539 publications with iNOS and Drug Toxicity
Neoplasms Antibodies	>381 publications with iNOS and Neoplasms
Lung Diseases Antibodies	>323 publications with iNOS and Lung Diseases
Reperfusion Injury Antibodies	>280 publications with iNOS and Reperfusion Injury
Liver Diseases Antibodies	>239 publications with iNOS and Liver Diseases
Intestinal Diseases Antibodies	>228 publications with iNOS and Intestinal Diseases
Endotoxemia Antibodies	>225 publications with iNOS and Endotoxemia
Shock, Septic Antibodies	>188 publications with iNOS and Shock, Septic

Organs/Tissues associated with anti-iNOS antibody

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>61 publications with iNOS and Connective Tissue
Ovary Antibodies	>36 publications with iNOS and Ovary
Placenta Antibodies	>30 publications with iNOS and Placenta
Uncharacterized Tissue Antibodies	>1 publications with iNOS and Uncharacterized Tissue