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Semaphorin-3a (Central Region) (SEMA3A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS472057

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect… More details

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Product Overview

Online Datasheet in PDF
Product Name
Semaphorin-3A (Central Region) (SEMA3A), Polyclonal Antibody
Full Product Name
Semaphorin-3A (Central Region)
Product Gene Name
anti-SEMA3A antibody
Product Synonym Names
Sema3A; Semaphorin III
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Purity/Purification
Affinity Purified
Specificity
The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A and neonatal rat brain lysates.
Preparation and Storage
Store at -20°C. Stable for 1 year.
Chromosome Location
Chromosome: 7; NC_000007.13 (83587659..83824217, complement). Location: 7p12.1
OMIM
603961
3D Structure
ModBase 3D Structure for Q14563
Immunogen
Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Sema3A. The sequence used is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.
Specificity Note
All molecular weights (MW) are confirmed by comparison to MW standards and to western blot mobilities of known proteins with similar MW.
"Native" western blot utilizes non-reducing sample buffer (no mercaptoethanol or SDS), normal SDS-PAGE gel electrophoresis, and no methanol in transfer buffers.
Applications Tested/Suitable for anti-SEMA3A antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-SEMA3A antibody
ELISA: 1:2000
Western Blot (WB): 1:1000
Immunohistochemistry (IHC): 1:300
Immunocytochemistry (ICC): 1:100
End user should determine optimal dilution for their particular applications and experiments. Western blot membranes were incubated with diluted antibody in 5% non-fat milk, Tris buffer, 0.04% Tween20 for 1 hour at room temperature.

Testing Data of anti-SEMA3A antibody

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A ( MBS472059 ) (lanes 1 & 2), anti-Sema3A (MBS472057) (lanes 3 & 4) and anti-Sema3A ( MBS472058 ) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.
Rabbit Anti-SEMA3A Polyclonal Antibody – Cat# MBS472057

Test Data of anti-SEMA3A antibody

Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (MBS472057) then anti-RabbitHRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Rabbit Anti-SEMA3A Polyclonal Antibody, Test Data – Cat# MBS472057

Product References and Citations for anti-SEMA3A antibody

• Kolodokin, A.L. et al. (1993) Cell 75:1389.
• Luo, Y. et al. (1993) Cell 75:217.
• Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
• Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068 (WB: R. E14 spinal cord)

Other Notes

Small volumes of anti-SEMA3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA3A purchase

MBS472057 is an antibody targeting SEMA3A. Also known as Semaphorin-3A (Central Region). Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA3A. It may not necessarily be applicable to this product.

NCBI GI #
5174673
NCBI GeneID
10371
NCBI Accession #
NP_006071.1
UniProt Primary Accession #
Q14563
UniProt Related Accession #
Q14563; Q75MQ2; Q86UJ2
GenBank Nucleotide #
NM_006080.2
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
NCBI Official Symbol
SEMA3A
NCBI Official Synonym Symbols
SemD; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243
UniProt Protein Name
Semaphorin-3A
UniProt Synonym Protein Names
Semaphorin III
Protein Family
Semaphorin
UniProt Gene Name
SEMA3A
UniProt Synonym Gene Names
SEMAD
UniProt Entry Name
SEM3A_HUMAN
NCBI Protein Information
semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; OTTHUMP00000161379; OTTHUMP00000208884

NCBI Summary for SEMA3A

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq]
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UniProt Comments for SEMA3A

Function: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1

Subcellular location: Secreted

Domain: Strong binding to neuropilin is mediated by the carboxy third of the protein.

Sequence similarities: Belongs to the semaphorin family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 PSI domain.Contains 1 Sema domain.
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Research Articles on SEMA3A

1. Both systemic and tumor-targeted delivery of SEMA3A inhibits tumor angiogenesis and tumor growth in multiple mouse models; moreover, SEMA3A inhibits the metastatic spreading from primary tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SEMA3A antibody

 Reference Product  PubMed Publications
 NRP1 antibody  >38 publications with SEMA3A and NRP1
 SEMA3B antibody  >6 publications with SEMA3A and SEMA3B

Pathways associated with anti-SEMA3A antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CRMPs In Sema3A Signaling Pathway antibodies  CRMPs In Sema3A Signaling Pathway Diagram
 SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway antibodies  SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway Diagram
 Sema3A PAK Dependent Axon Repulsion Pathway antibodies  Sema3A PAK Dependent Axon Repulsion Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram

Diseases associated with anti-SEMA3A antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >49 publications with SEMA3A and Neoplasms
 Carcinoma Antibodies  >13 publications with SEMA3A and Carcinoma
 Lung Neoplasms Antibodies  >10 publications with SEMA3A and Lung Neoplasms
 Adenocarcinoma Antibodies  >9 publications with SEMA3A and Adenocarcinoma
 Inflammation Antibodies  >8 publications with SEMA3A and Inflammation
 Congenital Abnormalities Antibodies  >7 publications with SEMA3A and Congenital Abnormalities
 Breast Neoplasms Antibodies  >6 publications with SEMA3A and Breast Neoplasms
 Drug Toxicity Antibodies  >6 publications with SEMA3A and Drug Toxicity
 Glioma Antibodies  >5 publications with SEMA3A and Glioma
 Heart Defects, Congenital Antibodies  >4 publications with SEMA3A and Heart Defects, Congenital

Organs/Tissues associated with anti-SEMA3A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >228 publications with SEMA3A and Brain
 Embryonic Tissue Antibodies  >133 publications with SEMA3A and Embryonic Tissue
 Blood Antibodies  >72 publications with SEMA3A and Blood
 Eye Antibodies  >47 publications with SEMA3A and Eye
 Muscle Antibodies  >31 publications with SEMA3A and Muscle
 Kidney Antibodies  >26 publications with SEMA3A and Kidney
 Lung Antibodies  >23 publications with SEMA3A and Lung
 Heart Antibodies  >21 publications with SEMA3A and Heart
 Skin Antibodies  >15 publications with SEMA3A and Skin
 Connective Tissue Antibodies  >10 publications with SEMA3A and Connective Tissue
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