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Actin (ACTB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS474080

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four alpha isoforms: skeletal, cardiac, and two smooth… More details

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Product Overview

Online Datasheet in PDF
Product Name
Actin (ACTB), Polyclonal Antibody
Full Product Name
Actin (Tyr-53), Phospho-Specific
Product Gene Name
anti-ACTB antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken
Purity/Purification
Affinity Purified
Specificity
This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.

All molecular weights (MW) are confirmed by comparison to MW standards and to western blot mobilites of known proteins with similar MW. "Native" western blots utilizes non-reducing sample buffer (no mercaptoethanol or SDS), normal SDS-PAGE gel electrophoresis, and no methanol in transfer buffers.
Preparation and Storage
Store at -20°C. Stable for 1 year.
OMIM
102630
3D Structure
ModBase 3D Structure for P60709
Buffer
Rabbit polyclonal, affinity-purified antibody is supplied in 100 uL phosphate buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
Immunogen
Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.
Applications Tested/Suitable for anti-ACTB antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes for anti-ACTB antibody
WB: 1:1000
ICC: 1:50
EIA: 1:2000

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-ACTB antibody

Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).
Rabbit Anti-ACTB Polyclonal Antibody – Cat# MBS474080

Immunocytochemistry (ICC) of anti-ACTB antibody

Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (MBS474001) or phospho-Actin (Tyr-53) peptide, respectively (right).
Rabbit Anti-ACTB Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS474080

Product References and Citations for anti-ACTB antibody

Vonach, C. et al. (2011) British J Cancer. 105:263. Jungbluth, A. et al. (1995) FEBS Let. 375:87.Baba, T. et al. (2003) J. Immunol. 170: 3762.Winder, S.J. et al. (2005) J. Cell Sci. 118:651.Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS474080 is an antibody targeting ACTB. Also known as Actin (Tyr-53), Phospho-Specific. Reactive in Human, Rat, Mouse, Chicken. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
4501885
NCBI GeneID
60
NCBI Accession #
NP_001092.1
UniProt Primary Accession #
P60709
UniProt Related Accession #
P60709
GenBank Nucleotide #
NM_001101.5
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
Actin
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
NCBI Protein Information
actin, cytoplasmic 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
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Research Articles on ACTB

1. Report recurrent GLI1-ACTB gene fusions in a group of malignant mesenchymal neoplasms involving soft tissue, and occasionally bone, with an often nested epithelioid phenotype and strong S100 immunoreactivity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACTB antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram

Diseases associated with anti-ACTB antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >83 publications with ACTB and Neoplasms
 Carcinoma Antibodies  >39 publications with ACTB and Carcinoma
 Liver Diseases Antibodies  >17 publications with ACTB and Liver Diseases
 Disease Models, Animal Antibodies  >16 publications with ACTB and Disease Models, Animal
 Brain Diseases Antibodies  >13 publications with ACTB and Brain Diseases
 Lung Diseases Antibodies  >12 publications with ACTB and Lung Diseases
 Necrosis Antibodies  >12 publications with ACTB and Necrosis
 Liver Neoplasms Antibodies  >11 publications with ACTB and Liver Neoplasms
 Kidney Diseases Antibodies  >11 publications with ACTB and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >9 publications with ACTB and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-ACTB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >54 publications with ACTB and Blood
 Brain Antibodies  >46 publications with ACTB and Brain
 Liver Antibodies  >43 publications with ACTB and Liver
 Muscle Antibodies  >40 publications with ACTB and Muscle
 Kidney Antibodies  >24 publications with ACTB and Kidney
 Lung Antibodies  >20 publications with ACTB and Lung
 Bone Antibodies  >18 publications with ACTB and Bone
 Heart Antibodies  >18 publications with ACTB and Heart
 Embryonic Tissue Antibodies  >14 publications with ACTB and Embryonic Tissue
 Skin Antibodies  >13 publications with ACTB and Skin
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