Rabbit Anti-ARPC1B/p41-ARC Polyclonal Antibody – Cat# MBS474084

Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).

ARPC1B/p41-ARC Antibody – Rabbit Polyclonal

Name: ARPC1B/p41-ARC Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS474084
Price: 500 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS474084

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Arpc1b/p41-Arc, Polyclonal Antibody

Full Product Name

Arpc1b/p41-Arc (Thr-21), Phospho-Specific

Product Gene Name

anti-Arpc1b/p41-Arc antibody

Product Synonym Names

Arc41; p41; Arpc1b

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Rat, Mouse, Chicken, Frog

Form/Format

Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Purity/Purification

Affinity Purified

Specificity

This antibody was affinity purified using phospho-Arpc1b (Thr-21) peptide (without carrier). The antibody detects a 41 kDa* protein corresponding to the molecular mass of Arpc1b on SDS-PAGE immunoblots of human A431 and mouse C2C12 treated with calyculin A. This reactivity is removed with lambda phosphatase treatment.

Preparation and Storage

Store at -20 degree C. Stable for 1 year.

Molecular Weight

41 kDa

OMIM

604223

3D Structure

ModBase 3D Structure for O15143

Immunogen

Phospho-Arpc1b/p41-Arc (Thr-21) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 21 in human Arpc1b. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arpc1b, and has some homology to the conserved site in isoform Arpc1a.

Applications Tested/Suitable for anti-Arpc1b/p41-Arc antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-Arpc1b/p41-Arc antibody

ELISA: 1:1000
WB: 1:500
WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Product References and Citations for anti-Arpc1b/p41-Arc antibody

Kim, Y.B. et al. (2015) J Biol Chem. 290(34):21076 WB: PC3 cellsHo, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. Welch, M.D. et al. (1997) J Cell Biol. 138(2):375.Vadlamudi, R.K. et al. (2004) EMBO Rep. 5(2):154.Molli, P.R. et al. (2010) J Cell Biol. 190(1):101.

Other Notes

Small volumes of anti-Arpc1b/p41-Arc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Arpc1b/p41-Arc purchase

MBS474084 is an antibody targeting Arpc1b/p41-Arc. Also known as Arpc1b/p41-Arc (Thr-21), Phospho-Specific. Reactive in Human, Rat, Mouse, Chicken, Frog. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Arpc1b/p41-Arc. It may not necessarily be applicable to this product.

NCBI GI #

5031601

NCBI GeneID

10095

NCBI Accession #

NP_005711.1

UniProt Primary Accession #

O15143

UniProt Related Accession #

O15143

GenBank Nucleotide #

NM_005720.4

NCBI Official Full Name

actin-related protein 2/3 complex subunit 1B

NCBI Official Synonym Full Names

actin related protein 2/3 complex subunit 1B

NCBI Official Symbol

ARPC1B

NCBI Official Synonym Symbols

ARC41; PLTEID; p40-ARC; p41-ARC

UniProt Protein Name

Actin-related protein 2/3 complex subunit 1B

UniProt Synonym Protein Names

Arp2/3 complex 41 kDa subunit; p41-ARC

UniProt Gene Name

ARPC1B

UniProt Synonym Gene Names

ARC41

UniProt Entry Name

ARC1B_HUMAN

NCBI Protein Information

actin-related protein 2/3 complex subunit 1B

NCBI Summary for Arpc1b/p41-Arc

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. This protein also has a role in centrosomal homeostasis by being an activator and substrate of the Aurora A kinase. [provided by RefSeq, Mar 2011]

UniProt Comments for Arpc1b/p41-Arc

ARPC1B: Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Belongs to the WD repeat ARPC1 family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: Arp2/3 protein complex; focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function: actin filament binding; structural constituent of cytoskeleton

Biological Process: cell motility

Research Articles on Arpc1b/p41-Arc

1. A homozygous frameshift mutation in ARPC1B (p.Val91Trpfs*30) was identified in a child with microthrombocytopenia, eosinophilia and inflammatory disease. Platelet lysates contained no ARPC1B protein and reduced Arp2/3 complex. Missense ARPC1B mutations were identified in an unrelated patient with similar symptoms and ARPC1B deficiency. ARPC1B-deficient platelets are microthrombocytes with abnormal spreading behavior.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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