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MEMO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS474122

During cell migration, actin assembly drives cell membrane protrusion, while microtubules (MTs) extend within protrusions to promote adhesion site turnover. Memo (mediator of ErbB2-driven cell motility) is an effector of the ErbB2 receptor tyrosine kinase… More details

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Product Overview

Online Datasheet in PDF
Product Name
Memo (MEMO1), Polyclonal Antibody
Full Product Name
Memo (N-Terminal Region)
Product Gene Name
anti-MEMO1 antibody
Product Synonym Names
CGI27; c21orf19like; NS5ATP7
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken
Form/Format
Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Purity/Purification
Affinity Purified
Specificity
This antibody was affinity purified using Memo (N-terminal region) peptide (without carrier). The antibody detects a significant 33 kDa* protein corresponding to the molecular mass of Memo on SDS-PAGE immunoblots of mouse heart, mouse C2C12, and rabbit spleen fibroblasts.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Molecular Weight
33 kDa
OMIM
611786
3D Structure
ModBase 3D Structure for Q9Y316
Immunogen
Memo synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human Memo. This peptide sequence is highly conserved in rat, mouse, and chicken Memo.
Applications Tested/Suitable for anti-MEMO1 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes for anti-MEMO1 antibody
ELISA: 1:2000
ICC: 1:200
WB: 1:1000
WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Western Blot (WB) of anti-MEMO1 antibody

Western blot analysis of Memo expression in adult mouse heart (lane 1 & 4), mouse C2C12 cells (lane 2 & 5), and rabbit spleen fibroblast cells (lane 3 & 6). The blot was probed with anti-Memo (N-terminal region) (MP3721; lanes 1-6) in the presence (lanes 4-6) or absence (lanes 1-3) of Memo blocking peptide (MX3725).
Rabbit Anti-MEMO1 Polyclonal Antibody – Cat# MBS474122

Immunocytochemistry (ICC) of anti-MEMO1 antibody

Immunocytochemical labeling of Memo in rabbit spleen fibroblasts that were fixed in paraformaldehyde and permeabilized with NP-40. The cells were probed with the Memo (N-terminal Region) MP3721, then the antibody was detected using goat anti-rabbit DyLight 594. The antibody was used in the absence (left) or presence (right) of it's blocking peptide (MX3725).
Rabbit Anti-MEMO1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS474122

Product References and Citations for anti-MEMO1 antibody

Qiu, C. et al. (2008) J. Biol. Chem. 283:2734.Zaoui, K. et al. (2008) J. Cell Biol. 183:401.Meira, M. et al. (2009) J. Cell Sci. 122:787.

Other Notes

Small volumes of anti-MEMO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MEMO1 purchase

MBS474122 is an antibody targeting MEMO1. Also known as Memo (N-Terminal Region). Reactive in Human, Rat, Mouse, Chicken. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MEMO1. It may not necessarily be applicable to this product.

NCBI GI #
212286159
NCBI GeneID
51072
NCBI Accession #
NP_001131074.1
UniProt Primary Accession #
Q9Y316
UniProt Related Accession #
Q9Y316
GenBank Nucleotide #
NM_001137602.2
NCBI Official Full Name
protein MEMO1 isoform 2
NCBI Official Synonym Full Names
mediator of cell motility 1
NCBI Official Symbol
MEMO1
NCBI Official Synonym Symbols
MEMO; C2orf4; CGI-27; NS5ATP7
UniProt Protein Name
Protein MEMO1
UniProt Synonym Protein Names
C21orf19-like protein; Hepatitis C virus NS5A-transactivated protein 7; HCV NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Mediator of cell motility 1; Memo-1
Protein Family
Protein
UniProt Gene Name
MEMO1
UniProt Synonym Gene Names
C2orf4; MEMO; NS5ATP7; HCV NS5A-transactivated protein 7; Mediator of cell motility 1; Memo-1
UniProt Entry Name
MEMO1_HUMAN
NCBI Protein Information
protein MEMO1

UniProt Comments for MEMO1

MEMO1: a highly conserved protein that controls cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Regulates directionality during HER2-dependent cell migration. Controls microtubules, the actin network, and adhesion site formation in migrating cells via the MEMO1-RHOA-DIAPH1 signaling pathway. Homologous to class III nonheme iron-dependent dioxygenases. No metal-binding or enzymatic activity has been detected. Binds HER2 phosphorylated at Y1222 via its vestigial enzymatic active site. Binds to and influences insulin-like growth factor-I (IRS1) receptor-dependent signaling events in breast carcinogenesis. Influences the epithelial-mesenchymal transition (EMT) via the IRS1/PI3K/Akt/Snail1 pathway. Interacts with cofilin, and influences PLCg1 and cofilin activities. Increased expression is associated with cancer aggressiveness. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Two isoform of the human protein are produced by alternatuve splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: nucleus; cytosol

Molecular Function: protein binding

Biological Process: regulation of microtubule-based process
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Research Articles on MEMO1

1. Study identify Memo as an important key mediator between the heregulin and estrogen signaling pathways, which affects both breast cancer cell migration and proliferation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MEMO1 antibody

 Reference Product  PubMed Publications
 ERBB2 antibody  >9 publications with MEMO1 and ERBB2

Organs/Tissues associated with anti-MEMO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with MEMO1 and Lung
 Blood Antibodies  >1 publications with MEMO1 and Blood
 Mammary Gland Antibodies  >1 publications with MEMO1 and Mammary Gland
 Eye Antibodies  >1 publications with MEMO1 and Eye
 Skin Antibodies  >1 publications with MEMO1 and Skin
 Brain Antibodies  >1 publications with MEMO1 and Brain
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