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Semaphorin-3a (SEMA3A) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS474141

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect… More details

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Product Overview

Online Datasheet in PDF
Product Name
Semaphorin-3A (SEMA3A), Monoclonal Antibody
Full Product Name
Semaphorin-3A (Central Region)
Product Gene Name
Product Synonym Names
Sema3A; Semaphorin III
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Rat, Mouse
Form/Format
Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Purity/Purification
Affinity Purified
Specificity
This antibody was affinity purified using Semaphorin 3A (Central region) peptide (without carrier). The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A and adult mouse brain lysates.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Molecular Weight
95 kDa
3D Structure
ModBase 3D Structure for Q14563
Immunogen
Clone M317 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Sema3A. The sequence used is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.
Applications Tested/Suitable for anti-SEMA3A antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-SEMA3A antibody
ELISA: 1:1000
WB: 1:500
WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Western Blot (WB) of anti-SEMA3A antibody

Western blots of human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 1-4). The blots were probed with rabbit polyclonal Sema3A (Central region) at 1:250 (lane 1) and 1:1000 (lane 2) and mouse monoclonal Sema3A (Central region) at 1:250 (lane 3) and 1:1000 (lane 4). Both antibodies recognize the 95 kDa and 125 kDa forms of the recombinant Sema3A.
Mouse Anti-SEMA3A Monoclonal Antibody – Cat# MBS474141

Product References and Citations for anti-SEMA3A antibody

Kolodkin, A.L. et al. (1993) Cell 75:1389. Luo, Y. et al. (1993) Cell 75:217. Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.

Other Notes

Small volumes of anti-SEMA3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA3A purchase

MBS474141 is an antibody targeting SEMA3A. Also known as Semaphorin-3A (Central Region). Reactive in Human, Rat, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA3A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
semaphorin 3A
NCBI Official Symbol
SEMA3A
NCBI Official Synonym Symbols
HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
UniProt Protein Name
Semaphorin-3A
UniProt Synonym Protein Names
Semaphorin III; Sema III
Protein Family
UniProt Gene Name
SEMA3A
UniProt Synonym Gene Names
SEMAD; Sema III
NCBI Protein Information
semaphorin-3A

NCBI Summary for SEMA3A

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for SEMA3A

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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