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Protein Phosphatase 4c (PPP4C) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475007

Entrez Summary: Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X… More details

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Product Overview

Online Datasheet in PDF
Product Name
Protein Phosphatase 4C (PPP4C), Monoclonal Antibody
Full Product Name
Anti-Protein Phosphatase 4C Mouse mAb
Product Gene Name
Product Synonym Gene Name
PP4; PPX; PP4C; PPH3; PPP4
Product Synonym Names
PP X; PP-X; PP4; PP4C; PP4C; PP4C_HUMAN; PPH3; PPP4; ppp4c; PPX; protein phosphatase 4 (formerly X), catalytic subunit; Protein phosphatase 4 catalytic subunit; Protein phosphatase X; Protein phosphatase X; protein phosphatase X, catalytic subunit; Serine/threonine protein phosphatase 4 catalytic subunit; Serine/threonine-protein phosphatase 4 catalytic subunit
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2F11-D10-G4
Host
Mouse
Source: Human
Species Reactivity
Human
Sensitivity
This antibody detects endogenous levels of Protein Phosphatase 4C and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
0.8mg/ml (lot specific)
Molecular Weight
34
OMIM
X70218 mRNA
3D Structure
ModBase 3D Structure for P60510
Immunogen
Purified recombinant human Protein Phosphatase 4C protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily
Subunit Structure
Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3.
Subcellular Location
Cytoplasm. Nucleus
Applications Tested/Suitable for anti-PPP4C antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-PPP4C antibody
WB: 1:200
ICC: 1:200

Western Blot (WB) of anti-PPP4C antibody

Western blot detection of Protein Phosphatase 4C in Hela and Jurkat cell lysates using Protein Phosphatase 4C mouse mAb (1:200 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
Mouse Anti-PPP4C Monoclonal Antibody – Cat# MBS475007

Immunocytochemistry (ICC) of anti-PPP4C antibody

Immunocytochemistry of HeLa cells using anti-Protein Phosphatase 4C mouse mAb diluted 1:200.
Mouse Anti-PPP4C Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS475007

Immunohistochemistry (IHC) of anti-PPP4C antibody

IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C mouse mAb diluted 1/500-1/1000.
Mouse Anti-PPP4C Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS475007

Other Notes

Small volumes of anti-PPP4C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP4C purchase

MBS475007 is an antibody targeting PPP4C. Also known as Anti-Protein Phosphatase 4C Mouse mAb. Reactive in Human. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP4C. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein phosphatase 4 catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 4 catalytic subunit
NCBI Official Symbol
PPP4C
NCBI Official Synonym Symbols
PP4; PPX; PP-X; PP4C; PPH3; PPP4
UniProt Protein Name
Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Synonym Protein Names
Protein phosphatase X; PP-X
UniProt Gene Name
PPP4C
UniProt Synonym Gene Names
PPP4; PPX; PP4C; Pp4; PP-X
NCBI Protein Information
serine/threonine-protein phosphatase 4 catalytic subunit

UniProt Comments for PPP4C

PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane

Molecular Function: protein binding; protein serine/threonine phosphatase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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