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MAP2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475033

Entrez Summary: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in… More details

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Product Overview

Product Name
MAP2, Monoclonal Antibody
Full Product Name
Anti-MAP2 (N-term) Mouse mAb
Product Gene Name
Product Synonym Gene Name
MAP2A; MAP2B; MAP2C
Product Synonym Names
DKFZp686I2148; Dendrite specific MAP; DKFZp686I2148; MAP 2; MAP dendrite specific; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; Mtap 2; MTAP2_HUMAN
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1D7-B9-C8-F6
Host
Mouse
Source: Human
Species Reactivity
Human, Mouse
Sensitivity
This antibody detects endogenous levels of MAP2 (N-terminus) and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl)with 0.2%sodium azide, 0.1%BSA, 50%glycerol.
Purity/Purification
Affinity Purified
Concentration
1mg/ml (lot specific)
Molecular Weight
300
OMIM
U01828 mRNA
3D Structure
ModBase 3D Structure for P11137
Immunogen
Purified recombinant human MAP2 (N-terminus) protein fragments expressed in E Coli
Sequence Similarities
Contains 3 Tau/MAP repeats.
Subcellular Location
Cytoplasm >cytoskeleton
Applications Tested/Suitable for anti-MAP2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-MAP2 antibody
WB: 1:1000
ICC: 1:150

Western Blot (WB) of anti-MAP2 antibody

Western blot detection of MAP2 (N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.
Mouse Anti-MAP2 Monoclonal Antibody – Cat# MBS475033

Immunocytochemistry (ICC) of anti-MAP2 antibody

Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-MAP2 (N-term) mouse mAb (dilution 1:100).
Mouse Anti-MAP2 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS475033

Immunoprecipitation (IP) of anti-MAP2 antibody

Immunoprecipitation analysis of Hela cell lysates using MAP2 (N-terminus) mouse mAb.
Mouse Anti-MAP2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475033

Other Notes

Small volumes of anti-MAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP2 purchase

MBS475033 is an antibody targeting MAP2. Also known as Anti-MAP2 (N-term) Mouse mAb. Reactive in Human, Mouse. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
microtubule-associated protein 2 isoform 5
NCBI Official Synonym Full Names
microtubule associated protein 2
NCBI Official Symbol
MAP2
NCBI Official Synonym Symbols
MAP2A; MAP2B; MAP2C
UniProt Protein Name
Microtubule-associated protein 2
UniProt Gene Name
MAP2
UniProt Synonym Gene Names
MAP-2
NCBI Protein Information
microtubule-associated protein 2

NCBI Summary for MAP2

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
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UniProt Comments for MAP2

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: cytoplasm; microtubule associated complex; neuron projection; nucleolus

Molecular Function: microtubule binding; protein binding

Biological Process: central nervous system neuron development; dendrite morphogenesis; microtubule cytoskeleton organization and biogenesis; neurite development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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