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Beta-Actin (ACTB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475054

Entrez Summary: This gene encodes one of six different actin proteins.Actins are highly conserved proteins that are involved in cell motility, structure, and integrity.This actin is a major constituent of the contractile apparatus and one of the two nonmuscle… More details

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Product Overview

Online Datasheet in PDF
Product Name
beta-Actin (ACTB), Monoclonal Antibody
Full Product Name
Anti-beta-Actin Mouse mAb
Product Gene Name
anti-ACTB antibody
Product Synonym Gene Name
BRWS1; PS1TP5BP1
Product Synonym Names
A X actin like protein; ACTB; ACTB_HUMAN; Actin; Actin cytoplasmic 1; Actx; b actin; b-actin; Beta cytoskeletal actin; Beta-actin; cytoplasmic 1; Melanoma X actin; N-terminally processed; PS1TP5 binding protein 1; PS1TP5BP1
Clonality
Monoclonal
Isotype
IgG3
Clone Number
6D7-F6-G10
Host
Mouse
Source: Human
Species Reactivity
Human, Rat, Mouse, Goat, Monkey, Hamster
Sensitivity
This antibody detects endogenous levels of beta actin and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal in PB (pH 7.4) containing with 0.02% sodium azide, 50% glycerol and 1mg/ml BSA.
Purity/Purification
Affinity Purified
Concentration
0.5mg/ml (lot specific)
Molecular Weight
45
OMIM
X00351 mRNA
3D Structure
ModBase 3D Structure for P60709
Immunogen
Purified recombinant human beta actin protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the actin family.
Subunit Structure
Polymerization of globular actin (G-actin)leads to a structural filament (F-actin)in the form of a two-stranded helix.Each actin can bind to 4 others.Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C.In muscle cells, the BAF complex also contains DPF3.Found in a complex with XPO6, Ran, ACTB and PFN1.Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT.Interacts with XPO6 and EMD.Interacts with ERBB2.Interacts with GCSAM.
Subcellular Location
Cytoplasm; cytoskeleton
Applications Tested/Suitable for anti-ACTB antibody
Western Blot (WB)
Application Notes for anti-ACTB antibody
WB: 1:1500

Western Blot (WB) of anti-ACTB antibody

Western blot detection of beta actin in Hela, 3T3, COS7, CHO-k1 and Goat muscle cell lysates using beta actin mouse mAb (1:1500 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Mouse Anti-ACTB Monoclonal Antibody – Cat# MBS475054

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS475054 is an antibody targeting ACTB. Also known as Anti-beta-Actin Mouse mAb. Reactive in Human, Rat, Mouse, Goat, Monkey, Hamster. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
4501885
NCBI GeneID
60
NCBI Accession #
NP_001092.1
UniProt Primary Accession #
P60709
UniProt Secondary Accession #
P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
UniProt Related Accession #
P60709
GenBank Nucleotide #
NM_001101
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
Actin
UniProt Gene Name
ACTB
NCBI Protein Information
actin, cytoplasmic 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTB

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; membrane; NuA4 histone acetyltransferase complex; nuclear chromatin; nucleoplasm; plasma membrane; protein complex; ribonucleoprotein complex

Molecular Function: identical protein binding; kinesin binding; nitric-oxide synthase binding; nucleosomal DNA binding; protein binding; structural constituent of cytoskeleton; Tat protein binding

Biological Process: ATP-dependent chromatin remodeling; cell motility; ephrin receptor signaling pathway; positive regulation of gene expression, epigenetic; retinal homeostasis; substantia nigra development; vascular endothelial growth factor receptor signaling pathway

Disease: Baraitser-winter Syndrome 1; Dystonia, Juvenile-onset
Show more

Research Articles on ACTB

1. crucial role of Glu270 in ADP-ribosylation of actin by Ia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACTB antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram

Diseases associated with anti-ACTB antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >83 publications with ACTB and Neoplasms
 Carcinoma Antibodies  >39 publications with ACTB and Carcinoma
 Liver Diseases Antibodies  >17 publications with ACTB and Liver Diseases
 Disease Models, Animal Antibodies  >16 publications with ACTB and Disease Models, Animal
 Brain Diseases Antibodies  >13 publications with ACTB and Brain Diseases
 Lung Diseases Antibodies  >12 publications with ACTB and Lung Diseases
 Necrosis Antibodies  >12 publications with ACTB and Necrosis
 Liver Neoplasms Antibodies  >11 publications with ACTB and Liver Neoplasms
 Kidney Diseases Antibodies  >11 publications with ACTB and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >9 publications with ACTB and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-ACTB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >54 publications with ACTB and Blood
 Brain Antibodies  >46 publications with ACTB and Brain
 Liver Antibodies  >43 publications with ACTB and Liver
 Muscle Antibodies  >40 publications with ACTB and Muscle
 Kidney Antibodies  >24 publications with ACTB and Kidney
 Lung Antibodies  >20 publications with ACTB and Lung
 Bone Antibodies  >18 publications with ACTB and Bone
 Heart Antibodies  >18 publications with ACTB and Heart
 Embryonic Tissue Antibodies  >14 publications with ACTB and Embryonic Tissue
 Skin Antibodies  >13 publications with ACTB and Skin
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