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HSP70 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475097

Entrez Summary: This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Hsp70, Monoclonal Antibody
Full Product Name
Anti-Hsp70 (N-term) Mouse mAb
Product Gene Name
Product Synonym Gene Name
hspa1a; HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
Product Synonym Names
DAQB 147D11.1 001; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A/1B; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70-1/HSP70-2; HSP70-1A; HSP70.1; HSP70.1/HSP70.2; HSP70I; HSP71_HUMAN; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2D4-F7-C11
Host
Mouse
Source: Human
Species Reactivity
Human
Sensitivity
This antibody detects endogenous levels of Hsp70 (N-terminus) and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Purity/Purification
Affinity Purified
Concentration
1mg/ml (lot specific)
Molecular Weight
70
3D Structure
ModBase 3D Structure for P08107
Immunogen
Purified recombinant human Hsp70 (N-terminus) protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the heat shock protein 70 family
Subunit Structure
Component of a complex composed at least of CATSPER1, CATSPERB, CATSPERG and HSPA1B. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3.
Subcellular Location
Cytoplasm
Applications Tested/Suitable for anti-Hsp70 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Hsp70 antibody
WB: 1:1000
ICC: 1:300

Western Blot (WB) of anti-Hsp70 antibody

Western blot detection of Hsp70 (N-terminus) in Heat shcok Hela, Hela and HepG2 lysates using Hsp70 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 70KDa.Observed band size: 70KDa.
Mouse Anti-HSP70 Monoclonal Antibody – Cat# MBS475097

Immunocytochemistry (ICC) of anti-Hsp70 antibody

Immunocytochemistry stain of Hela using Hsp70 (N-terminus) mouse mAb (1:300).
Mouse Anti-HSP70 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS475097

Other Notes

Small volumes of anti-Hsp70 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp70 purchase

MBS475097 is an antibody targeting Hsp70. Also known as Anti-Hsp70 (N-term) Mouse mAb. Reactive in Human. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp70. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 1B
NCBI Official Symbol
HSPA1B
NCBI Official Synonym Symbols
HSP70-2; HSP70.2; HSP70-1B
NCBI Protein Information
heat shock 70 kDa protein 1B

NCBI Summary for Hsp70

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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