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COX IV Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475127

Entrez Summary: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain.It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton… More details

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Product Overview

Product Name
COX IV, Monoclonal Antibody
Full Product Name
Anti-COX IV Mouse mAb
Product Gene Name
anti-COX IV antibody
Product Synonym Gene Name
COX4; COXIV; COX4-1.
Product Synonym Names
AL024441; COX 4; COX IV 1; COX IV; COX IV-1; Cox4; COX41_HUMAN; Cox4a; COX4B; COX4I1; COX4I2; COX4L2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome C Oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); Cytochrome c oxydase subunit; dJ857M17.2; MGC105470; MGC72016
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4D11-B3-E8
Host
Mouse
Source: Human
Species Reactivity
Human, Mouse, Rat, Hamster, Goat, Monkey
Sensitivity
This antibody detects endogenous levels of COX IV and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)with 0.2% sodium azide, 0.1mg/mlBSA and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
1mg/ml (lot specific)
Molecular Weight
17
OMIM
M21575 mRNA
3D Structure
ModBase 3D Structure for P13073
Immunogen
A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
Sequence Similarities
Belongs to the cytochrome c oxidase IV family.
Subcellular Location
Mitochondrion inner membrane
Applications Tested/Suitable for anti-COX IV antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-COX IV antibody
WB: 1:1000
ICC: 1:150
FC: 1:100

Western Blot (WB) of anti-COX IV antibody

Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.
Mouse Anti-COX IV Monoclonal Antibody – Cat# MBS475127

Immunocytochemistry (ICC) of anti-COX IV antibody

Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.
Mouse Anti-COX IV Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS475127

Immunoprecipitation (IP) of anti-COX IV antibody

Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.
Mouse Anti-COX IV Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475127

Immunohistochemistry (IHC) of anti-COX IV antibody

Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8, 1:50 diluted), showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer (pH6.0).
Mouse Anti-COX IV Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS475127

Flow Cytometry (FC/FACS) of anti-COX IV antibody

Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
Mouse Anti-COX IV Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS475127

Western Blot (WB) of anti-COX IV antibody

Western blot detection of COX IV in Goat muscle, CHO-k1, COS7, 3T3, Hela, C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
Mouse Anti-COX IV Monoclonal Antibody, Western Blot (WB) – Cat# MBS475127

Other Notes

Small volumes of anti-COX IV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COX IV purchase

MBS475127 is an antibody targeting COX IV. Also known as Anti-COX IV Mouse mAb. Reactive in Human, Mouse, Rat, Hamster, Goat, Monkey. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for COX IV. It may not necessarily be applicable to this product.

NCBI GI #
974005293
NCBI GeneID
1327
NCBI Accession #
NP_001305715.1
UniProt Primary Accession #
P13073
UniProt Secondary Accession #
Q6P666; B2R4J2; D3DUM7
UniProt Related Accession #
P13073
GenBank Nucleotide #
NM_001861
NCBI Official Full Name
cytochrome c oxidase subunit 4 isoform 1, mitochondrial isoform 1
NCBI Official Synonym Full Names
cytochrome c oxidase subunit 4I1
NCBI Official Symbol
COX4I1
NCBI Official Synonym Symbols
COX4; COXIV; COX4-1; COXIV-1; COX IV-1
UniProt Protein Name
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
UniProt Synonym Protein Names
Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1
Protein Family
Cytochrome c oxidase
UniProt Gene Name
COX4I1
UniProt Synonym Gene Names
COX4; COX IV-1
NCBI Protein Information
cytochrome c oxidase subunit 4 isoform 1, mitochondrial

NCBI Summary for COX IV

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
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UniProt Comments for COX IV

COX4I1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Ubiquitous. Belongs to the cytochrome c oxidase IV family.

Protein type: EC 1.9.3.1; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex IV; mitochondrion; nucleus

Molecular Function: protein binding

Biological Process: generation of precursor metabolites and energy; mitochondrial electron transport, cytochrome c to oxygen
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Research Articles on COX IV

1. We provide evidence that COX4-1 controls BMI1 expression via a redox mechanism

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-COX IV antibody

 Reference Product  PubMed Publications
 COX5B antibody  >2 publications with COX IV and COX5B
 COX5A antibody  >2 publications with COX IV and COX5A
 MT-CO2 antibody  >2 publications with COX IV and MT-CO2
 COX7C antibody  >1 publications with COX IV and COX7C
 COX6B1 antibody  >1 publications with COX IV and COX6B1

Pathways associated with anti-COX IV antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cytochrome C Oxidase Pathway antibodies  Cytochrome C Oxidase Pathway Diagram
 Cytochrome C Oxidase Pathway antibodies  Cytochrome C Oxidase Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram

Diseases associated with anti-COX IV antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >5 publications with COX IV and Fatty Liver
 Disease Models, Animal Antibodies  >3 publications with COX IV and Disease Models, Animal
 Death Antibodies  >2 publications with COX IV and Death
 Weight Loss Antibodies  >2 publications with COX IV and Weight Loss
 Cardiovascular Diseases Antibodies  >1 publications with COX IV and Cardiovascular Diseases
 Atrophy Antibodies  >1 publications with COX IV and Atrophy
 Heart Diseases Antibodies  >1 publications with COX IV and Heart Diseases
 Fibrosis Antibodies  >1 publications with COX IV and Fibrosis
 Liver Diseases Antibodies  >1 publications with COX IV and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with COX IV and Kidney Diseases

Organs/Tissues associated with anti-COX IV antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >11 publications with COX IV and Muscle
 Liver Antibodies  >8 publications with COX IV and Liver
 Blood Antibodies  >8 publications with COX IV and Blood
 Adipose Tissue Antibodies  >7 publications with COX IV and Adipose Tissue
 Brain Antibodies  >5 publications with COX IV and Brain
 Kidney Antibodies  >2 publications with COX IV and Kidney
 Heart Antibodies  >2 publications with COX IV and Heart
 Connective Tissue Antibodies  >2 publications with COX IV and Connective Tissue
 Skin Antibodies  >1 publications with COX IV and Skin
 Embryonic Tissue Antibodies  >1 publications with COX IV and Embryonic Tissue
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