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IgG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475172

Entrez Summary: Mouse Anti-rabbit IgG mAb reacts with rabbit IgG and can be used as secondary antibody for rabbit antibodies in western blotting assay and ELISA. More details

$525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, Monoclonal Antibody
Full Product Name
Anti-Rabbit IgG Mouse mAb
Product Gene Name
Product Synonym Names
Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5B2-F9-D6
Host
Mouse
Source: Rabbit
Species Reactivity
Rat
Sensitivity
This antibody detects rabbit IgG.
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
5.85mg/ml (lot specific)
Immunogen
Rabbit IgG
Subcellular Location
Secreted

Product Categories/Family for anti-IgG antibody

Applications Tested/Suitable for anti-IgG antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-IgG antibody
WB: 1:50000

Western Blot (WB) of anti-IgG antibody

Western blot detection of MEK-2 in THP-1, Jurkat, Raji, 3T3 and Hela cell lysates using 310001 (MEK-2 rabbit pAb, 1:1000 diluted), then incubated with 201069 (Rabbit IgG mAb, 1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.
Mouse Anti-IgG Monoclonal Antibody – Cat# MBS475172

Western Blot (WB) of anti-IgG antibody

Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using Mouse Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:1000 diluted).
Mouse Anti-IgG Monoclonal Antibody, Western Blot (WB) – Cat# MBS475172

Immunoprecipitation (IP) of anti-IgG antibody

Immunoprecipitation analysis of CHO-K1 cell lysates transfected with HA-tagged NGAL using HA-Tag Rabbit pAb (390001, 1:100 diluted) (lane 2). Lane 1 contains rabbit IgG IP control lysate. All lanes are probed with HA-Tag Rabbit pAb (390001, 1:1000 diluted) as the primary antibody. Panel A incubated with secondary antibodies Anti-Rabbit whole IgG, HRP-linked Antibody for detecting.Panel B incubated with Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:50000 diluted) and the Anti-mouse whole IgG, HRP-linked Antibody was used for detecting.
Mouse Anti-IgG Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475172

Other Notes

Small volumes of anti-IgG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG purchase

MBS475172 is an antibody targeting IgG. Also known as Anti-Rabbit IgG Mouse mAb. Reactive in Rat. Host: Mouse
Source: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IgG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
IgG
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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