Mouse Anti-RAD51 Monoclonal Antibody – Cat# MBS475181

Western blot detection of Rad51 in CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Rad51 mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.

RAD51 Antibody – Mouse Monoclonal

Name: RAD51 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS475181
Price: 525 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475181

Entrez Summary: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of… More details

$525 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Rad51, Monoclonal Antibody

Full Product Name

Anti-Rad51 Mouse mAb

Product Gene Name

anti-Rad51 antibody

Product Synonym Gene Name

RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930

Product Synonym Names

BRCA1/BRCA2 containing complex, subunit 5; BRCC 5; BRCC5; DNA repair protein RAD51 homolog 1; DNA repair protein rhp51; HRAD51; HsRad51; HsT16930; MRMV2; Rad 51; RAD51; RAD51 homolog (RecA homolog, E Coli) (S. cerevisiae); RAD51 homolog A; RAD51 homolog; RAD51 recombinase; RAD51, S. cerevisiae, homolog of; RAD51_HUMAN; RAD51A; RECA; RecA like protein; RecA, E Coli, homolog of; Recombination protein A

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

4H10-G5-H4

Host

Mouse
Source: Human

Species Reactivity

Human, Mouse, Rat, Monkey

Sensitivity

This antibody detects endogenous levels of Rad51 and does not cross-react with related proteins.

Form/Format

0.03% Proclin300 and 50% glycerol.

Molecular Weight

OMIM

D13804 mRNA

3D Structure

ModBase 3D Structure for Q06609

Immunogen

Recombinant protein of human Rad51.

Sequence Similarities

Belongs to the RecA family. RAD51 subfamily. Contains 1 HhH domain.

Subunit Structure

Forms linear homooligomers, giving rise to a RAD51 nucleoprotein filament, which is essential for strand-pairing reactions during DNA recombination. Interacts with BRCA1 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Interacts with the BCDX2 subcomplex RAD51C:RAD51B. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer. Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR. Interacts with SPIDR; the interaction is direct and recruits RAD51 to DNA damage sites. Interacts with FIGNL1 (via N-terminal one-half region); the interaction is direct. Interacts with RAD51AP1 (via C-terminal region); the interaction is direct. Interacts with NABP2, RPA1, PALB2 and RAD51. Interacts with SWI5/C9orf119, and at lower level with SFR1/MEIR5. Interacts with hyperphosphorylated RPA2; this interaction is necessary for efficient recruitment to chromatin in response to DNA damage. Interacts with SWSAP1; involved in homologous recombination repair. Interacts with PARPBP, BRCA2 and RECQL5; these interactions interfere with the formation of the RAD51-DNA homologous recombination structure.

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm; perinuclear region. Mitochondrion matrix. Cytoplasm; cytoskeleton; microtubule organizing center; centrosome

Product Categories/Family for anti-Rad51 antibody

Epigenetics and Nuclear Signaling; DNA/RNA; DNA Damage & Repair; Homologous Recomb

Applications Tested/Suitable for anti-Rad51 antibody

Western Blot (WB)

Application Notes for anti-Rad51 antibody

WB: 1:1000

Other Notes

Small volumes of anti-Rad51 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rad51 purchase

MBS475181 is an antibody targeting Rad51. Also known as Anti-Rad51 Mouse mAb. Reactive in Human, Mouse, Rat, Monkey. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for Rad51. It may not necessarily be applicable to this product.

NCBI GI #

256017143

NCBI GeneID

5888

NCBI Accession #

NP_001157741.1

UniProt Primary Accession #

Q06609

UniProt Secondary Accession #

Q6FHX9; Q6ZNA8; Q9BV60; B0FXP0; B2R8T6

UniProt Related Accession #

Q06609

GenBank Nucleotide #

NM_002875

NCBI Official Full Name

DNA repair protein RAD51 homolog 1 isoform 2

NCBI Official Synonym Full Names

RAD51 recombinase

NCBI Official Symbol

RAD51

NCBI Official Synonym Symbols

RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930

UniProt Protein Name

DNA repair protein RAD51 homolog 1

UniProt Synonym Protein Names

RAD51 homolog A

Protein Family

DNA repair protein

UniProt Gene Name

RAD51

UniProt Synonym Gene Names

RAD51A; RECA; HsRAD51; hRAD51

NCBI Protein Information

DNA repair protein RAD51 homolog 1

NCBI Summary for Rad51

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

UniProt Comments for Rad51

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).

Research Articles on Rad51

1. This study revealed statistically significant association between appearance of G/A genotype in position -4601 of RAD51 gene and Colorectal cancer (CRC) risk.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rad51 antibody

Reference Product	PubMed Publications
RAD52 antibody	>404 publications with Rad51 and RAD52
BRCA2 antibody	>402 publications with Rad51 and BRCA2
BRCA1 antibody	>328 publications with Rad51 and BRCA1
DMC1 antibody	>198 publications with Rad51 and DMC1
ATM antibody	>149 publications with Rad51 and ATM
RAD51C antibody	>102 publications with Rad51 and RAD51C
BLM antibody	>73 publications with Rad51 and BLM
FANCD2 antibody	>71 publications with Rad51 and FANCD2
RAD54L antibody	>44 publications with Rad51 and RAD54L
RPA1 antibody	>35 publications with Rad51 and RPA1

Pathways associated with anti-Rad51 antibody

Products by Pathway	Pathway Diagram
Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway antibodies	Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway Diagram
BARD1 Signaling Events Pathway antibodies	BARD1 Signaling Events Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram
Double-Strand Break Repair Pathway antibodies	Double-Strand Break Repair Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Homologous DNA Pairing And Strand Exchange Pathway antibodies	Homologous DNA Pairing And Strand Exchange Pathway Diagram
Homologous Recombination Repair Pathway antibodies	Homologous Recombination Repair Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram

Diseases associated with anti-Rad51 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>582 publications with Rad51 and Neoplasms
Breast Neoplasms Antibodies	>240 publications with Rad51 and Breast Neoplasms
Carcinoma Antibodies	>132 publications with Rad51 and Carcinoma
Anemia Antibodies	>113 publications with Rad51 and Anemia
Drug Toxicity Antibodies	>79 publications with Rad51 and Drug Toxicity
Ovarian Neoplasms Antibodies	>70 publications with Rad51 and Ovarian Neoplasms
Hematologic Diseases Antibodies	>67 publications with Rad51 and Hematologic Diseases
Adenocarcinoma Antibodies	>46 publications with Rad51 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>45 publications with Rad51 and Neoplasms, Experimental
Nervous System Diseases Antibodies	>40 publications with Rad51 and Nervous System Diseases

Organs/Tissues associated with anti-Rad51 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>102 publications with Rad51 and Blood
Lung Antibodies	>59 publications with Rad51 and Lung
Testis Antibodies	>56 publications with Rad51 and Testis
Ovary Antibodies	>35 publications with Rad51 and Ovary
Bone Antibodies	>32 publications with Rad51 and Bone
Brain Antibodies	>30 publications with Rad51 and Brain
Bone Marrow Antibodies	>28 publications with Rad51 and Bone Marrow
Prostate Antibodies	>24 publications with Rad51 and Prostate
Skin Antibodies	>23 publications with Rad51 and Skin
Liver Antibodies	>17 publications with Rad51 and Liver