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PKM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475183

Pyruvate kinase is a glycolytic enzyme that catalyzes the conversion of phosphoenolpyruvate to pyruvate. PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect. More details

$525 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PKM2, Monoclonal Antibody
Full Product Name
Anti-PKM2 Mouse mAb
Product Gene Name
anti-PKM2 antibody
Product Synonym Gene Name
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Product Synonym Names
CTHBP; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM_HUMAN; MGC3932; OIP 3; OIP-3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK muscle type; PK, muscle type; PK2; PK3; PKM; PKM2; pykm; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; pyruvate kinase PKM; Pyruvate kinase, muscle 2; TCB; THBP1; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone-binding protein 1; Tumor M2 PK; Tumor M2-PK
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1F2-F4-H11
Host
Mouse
Species Reactivity
Human, Mouse, Monkey
Form/Format
Liquid ; PBS (pH 7.4) containing 50% glycerol , 0.5% BSA, and 0.02% sodium azide, pH 7.3
Purity/Purification
Affinity Purified
Concentration
0.7mg/mL (lot specific)
Preparation and Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Molecular Weight
Calculated MW: 58 kDa ; Observed MW: 58 kDa
OMIM
M23725 mRNA
3D Structure
ModBase 3D Structure for P14618
Immunogen
Purified recombinant human PKM2 protein fragments expressed in E Coli.

Product Categories/Family for anti-PKM2 antibody

Signal Transduction; Metabolism; Energy Metabolism Cancer; Tumor biomarkers; Enzymes; Other Cancer; Cancer Metabolism; Metabolic signaling pathway; Metabolism of carbohydrates Metabolism; Pathways and
Applications Tested/Suitable for anti-PKM2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-PKM2 antibody
WB: 1/500-1/1000
IF: 1:50-1/200
IP: 1/20

Western Blot (WB) of anti-PKM2 antibody

Western blot detection of PKM2 in HCT116, COS7, K562, A549 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
Mouse Anti-PKM2 Monoclonal Antibody – Cat# MBS475183

Immunofluorescence (IF) of anti-PKM2 antibody

Immunofluorescent analysis of 3T3 cells fixed by anhydrous methanol for 2 h at -20 degree C and using anti-PKM2 mouse mAb (dilution 1:500).
Mouse Anti-PKM2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS475183

Immunoprecipitation (IP) of anti-PKM2 antibody

Immunoprecipitation analysis of Hela cell lysates using PKM2.
Mouse Anti-PKM2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475183

Other Notes

Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM2 purchase

MBS475183 is an antibody targeting PKM2. Also known as Anti-PKM2 Mouse mAb. Reactive in Human, Mouse, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.

NCBI GI #
332164775
NCBI GeneID
5315
NCBI Accession #
NP_001193725.1
UniProt Primary Accession #
P14618
UniProt Secondary Accession #
P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
UniProt Related Accession #
P14618
GenBank Nucleotide #
NM_002654
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
NCBI Protein Information
pyruvate kinase PKM

NCBI Summary for PKM2

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
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UniProt Comments for PKM2

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
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Research Articles on PKM2

1. PKM2 was involved in progression of ESCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PKM2 antibody

 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway antibodies  Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with anti-PKM2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >21 publications with PKM2 and Neoplasms
 Drug Toxicity Antibodies  >9 publications with PKM2 and Drug Toxicity
 Lung Diseases Antibodies  >8 publications with PKM2 and Lung Diseases
 Fibrosis Antibodies  >8 publications with PKM2 and Fibrosis
 Nervous System Diseases Antibodies  >8 publications with PKM2 and Nervous System Diseases
 Inflammation Antibodies  >6 publications with PKM2 and Inflammation
 Cardiovascular Diseases Antibodies  >6 publications with PKM2 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >5 publications with PKM2 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >4 publications with PKM2 and Liver Diseases
 Necrosis Antibodies  >4 publications with PKM2 and Necrosis

Organs/Tissues associated with anti-PKM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >49 publications with PKM2 and Brain
 Blood Antibodies  >35 publications with PKM2 and Blood
 Muscle Antibodies  >20 publications with PKM2 and Muscle
 Kidney Antibodies  >14 publications with PKM2 and Kidney
 Liver Antibodies  >13 publications with PKM2 and Liver
 Heart Antibodies  >10 publications with PKM2 and Heart
 Nerve Antibodies  >8 publications with PKM2 and Nerve
 Vascular Antibodies  >8 publications with PKM2 and Vascular
 Lung Antibodies  >7 publications with PKM2 and Lung
 Intestine Antibodies  >4 publications with PKM2 and Intestine
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