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PP2A Alpha and Beta (PP2Abeta) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475192

Entrez Summary: This gene encodes the phosphatase 2A catalytic subunit.Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division.It consists of a common heteromeric core… More details

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Product Overview

Product Name
PP2A alpha and beta (PP2Abeta), Monoclonal Antibody
Full Product Name
Anti-PP2A alpha and beta Mouse mAb
Product Gene Name
Product Synonym Gene Name
PP2CB; PP2A alpha and beta.
Product Synonym Names
PP2A beta; PP2A-beta; PP2AB_HUMAN; PP2Abeta; PP2CB; Ppp2cb; Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; Protein phosphatase 2 catalytic subunit beta isozyme; Protein phosphatase 2, catalytic subunit, beta isoform; Protein phosphatase 2A catalytic subunit beta isoform; Protein phosphatase type 2A catalytic subunit; Serine/threonine protein phosphatase 2A catalytic subunit beta; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2G2-H6-G11-E8
Host
Mouse
Source: Human
Species Reactivity
Human, Mouse, Rat
Sensitivity
This antibody detects endogenous levels of PP2A alpha and PP2A beta.
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Purity/Purification
Affinity Purified
Molecular Weight
36
OMIM
X12656 mRNA
3D Structure
ModBase 3D Structure for P62714
Immunogen
Purified recombinant human full length PP2A beta protein expressed in E Coli
Sequence Similarities
Belongs to the PPP phosphatase family.PP-1 subfamily.
Subunit Structure
Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD.Interacts with TBCD by similarity.PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits.Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.Binds PPME1.May indirectly interact with SGOL1, most probably through regulatory B56 subunits.Interacts with CTTNBP2NL.
Applications Tested/Suitable for anti-PP2Abeta antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-PP2Abeta antibody
WB: 1:2000

Western Blot (WB) of anti-PP2Abeta antibody

Western blot detection of PP2A alpha and beta in Hela, MCF7, C6 and 3T3 cell lysates using PP2A alpha and beta mouse mAb (1:2000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
Mouse Anti-PP2Abeta Monoclonal Antibody – Cat# MBS475192

Immunoprecipitation (IP) of anti-PP2Abeta antibody

Immunoprecipitation analysis of Hela cell lysates using PP2A alpha and beta mouse mAb.
Mouse Anti-PP2Abeta Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475192

Other Notes

Small volumes of anti-PP2Abeta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PP2Abeta purchase

MBS475192 is an antibody targeting PP2Abeta. Also known as Anti-PP2A alpha and beta Mouse mAb. Reactive in Human, Mouse, Rat. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for PP2Abeta. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
NCBI Official Synonym Full Names
protein phosphatase 2 catalytic subunit beta
NCBI Official Symbol
PPP2CB
NCBI Official Synonym Symbols
PP2CB; PP2Abeta
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
UniProt Gene Name
PPP2CB
UniProt Synonym Gene Names
PP2A-beta
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

NCBI Summary for PP2Abeta

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]
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UniProt Comments for PP2Abeta

PPP2CB: one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: cytosol; protein phosphatase type 2A complex

Molecular Function: protein binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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