Mouse Anti-BAX Monoclonal Antibody – Cat# MBS475223

Western blot detection of Bax in COS7, Raji, CEM and Hela cell lysates using Bax mouse mAb (1:1000 diluted). Predicted band size:20KDa. Observed band size:20KDa.

BAX Antibody – Mouse Monoclonal

Name: BAX Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS475223
Price: 525 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475223

Entrez Summary: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a… More details

$525 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bax, Monoclonal Antibody

Full Product Name

Anti-Bax Mouse mAb

Product Gene Name

anti-Bax antibody

Product Synonym Gene Name

BCL2L4

Product Synonym Names

Apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; BAX; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta.; Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; BAX_HUMAN; BAXA; Bcl-2-like protein 4; BCL2 associated X protein; BCL2 associated X protein omega; BCL2 associated X protein transcript variant delta2; Bcl2-L-4; BCL2L4; membrane isoform alpha

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

2B12-G6-H6

Host

Mouse
Source: Human

Species Reactivity

Human, Monkey

Sensitivity

This antibody detects endogenous levels of Bax and does not cross-react with related proteins.

Form/Format

Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity Purified

Concentration

5.5mg/ml (lot specific)

Molecular Weight

OMIM

L22473 mRNA

3D Structure

ModBase 3D Structure for Q07812

Immunogen

Purified recombinant human Bax protein fragments expressed in E Coli

Sequence Similarities

Belongs to the Bcl-2 family.

Subunit Structure

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X (L) BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X (L) Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Interacts with BOP/C22orf29.

Subcellular Location

Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm.

Product Categories/Family for anti-Bax antibody

Research Areas Cell Biology; Apoptosis; Intracellular; Associated Proteins Cancer; Invasion/microenvironment; Apoptosis; Bcl 2 family Metabolism; Pathways and Processes; Metabolism processes

Applications Tested/Suitable for anti-Bax antibody

Western Blot (WB)

Application Notes for anti-Bax antibody

WB: 1:1000

Other Notes

Small volumes of anti-Bax antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bax purchase

MBS475223 is an antibody targeting Bax. Also known as Anti-Bax Mouse mAb. Reactive in Human, Monkey. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for Bax. It may not necessarily be applicable to this product.

NCBI GI #

612149787

NCBI GeneID

581

NCBI Accession #

NP_001278357.1

UniProt Primary Accession #

Q07812

UniProt Secondary Accession #

P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1

UniProt Related Accession #

Q07812

GenBank Nucleotide #

NM_138761

NCBI Official Full Name

apoptosis regulator BAX isoform 1

NCBI Official Synonym Full Names

BCL2 associated X, apoptosis regulator

NCBI Official Symbol

BAX

NCBI Official Synonym Symbols

BCL2L4

UniProt Protein Name

Apoptosis regulator BAX

UniProt Synonym Protein Names

Bcl-2-like protein 4; Bcl2-L-4

Protein Family

Apoptosis regulator

UniProt Gene Name

BAX

UniProt Synonym Gene Names

BCL2L4; Bcl2-L-4

NCBI Protein Information

apoptosis regulator BAX

NCBI Summary for Bax

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for Bax

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Research Articles on Bax

1. Findings reveal a novel mechanism by which p53 utilizes TFIIS.h to selectively promote the transcriptional elongation of the bax gene, upsurging cell death in response to severe DNA damage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Bax antibody

Products by Pathway	Pathway Diagram
Activation, Translocation And Oligomerization Of BAX Pathway antibodies	Activation, Translocation And Oligomerization Of BAX Pathway Diagram
AhR Pathway antibodies	AhR Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram

Diseases associated with anti-Bax antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>2020 publications with Bax and Necrosis
Nervous System Diseases Antibodies	>1868 publications with Bax and Nervous System Diseases
Heart Diseases Antibodies	>1852 publications with Bax and Heart Diseases
Ischemia Antibodies	>1546 publications with Bax and Ischemia
Brain Diseases Antibodies	>1395 publications with Bax and Brain Diseases
Inflammation Antibodies	>1032 publications with Bax and Inflammation
Liver Diseases Antibodies	>970 publications with Bax and Liver Diseases
Breast Neoplasms Antibodies	>962 publications with Bax and Breast Neoplasms
Intestinal Neoplasms Antibodies	>839 publications with Bax and Intestinal Neoplasms
Neoplasms, Experimental Antibodies	>770 publications with Bax and Neoplasms, Experimental

Organs/Tissues associated with anti-Bax antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3174 publications with Bax and Blood
Brain Antibodies	>2456 publications with Bax and Brain
Liver Antibodies	>1749 publications with Bax and Liver
Lung Antibodies	>1338 publications with Bax and Lung
Bone Antibodies	>791 publications with Bax and Bone
Nerve Antibodies	>737 publications with Bax and Nerve
Prostate Antibodies	>723 publications with Bax and Prostate
Skin Antibodies	>518 publications with Bax and Skin
Stomach Antibodies	>503 publications with Bax and Stomach
Eye Antibodies	>402 publications with Bax and Eye