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GRAP2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475230

Entrez Summary: This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein… More details

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Product Overview

Online Datasheet in PDF
Product Name
GRAP2, Monoclonal Antibody
Full Product Name
Anti-GRAP2 Mouse mAb
Product Gene Name
Product Synonym Gene Name
P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
Product Synonym Names
Adapter protein GRID; GADS; GADS protein; GRAP-2; Grap2; GRAP2_HUMAN; GRB-2-like protein; GRB2-related adapter protein 2; GRB2-related adaptor protein 2; GRB2-related protein with insert domain; GRB2L; GRBLG; GRBX; Grf-40; Grf40 adapter protein; Grf40; GRID; Growth factor receptor-binding protein; Growth factor receptor-bound protein 2-related adaptor protein 2; GRPL; Hema; Hematopoietic cell-associated adapter protein GrpL; Hematopoietic cell-associated adaptor protein GRPL; Mona; p38; Protein GADS; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5C7-F4-E7
Host
Mouse
Source: Human
Species Reactivity
Human
Sensitivity
This antibody detects endogenous levels of GRAP2 and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
4.8mg/mL (lot specific)
Molecular Weight
38
OMIM
AF102694 mRNA
3D Structure
ModBase 3D Structure for O75791
Immunogen
Recombinant protein of human GRAP2.
Sequence Similarities
Belongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.
Subunit Structure
Interacts with phosphorylated LIME1 upon TCR activation (By similarity). Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23.
Subcellular Location
Nucleus. Cytoplasm. Endosome.
Applications Tested/Suitable for anti-GRAP2 antibody
Western Blot (WB)
Application Notes for anti-GRAP2 antibody
WB: 1:1000

Western Blot (WB) of anti-GRAP2 antibody

Western blot detection of GRAP2 in GRAP2 positive cell (CEM, Molt-4, Jurkat) and negative cell (3T3, K562, Hela) lysates using GRAP2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
Mouse Anti-GRAP2 Monoclonal Antibody – Cat# MBS475230

Other Notes

Small volumes of anti-GRAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRAP2 purchase

MBS475230 is an antibody targeting GRAP2. Also known as Anti-GRAP2 Mouse mAb. Reactive in Human. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for GRAP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
GRB2-related adapter protein 2 isoform 1
NCBI Official Synonym Full Names
GRB2-related adaptor protein 2
NCBI Official Symbol
GRAP2
NCBI Official Synonym Symbols
P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
UniProt Protein Name
GRB2-related adapter protein 2
UniProt Synonym Protein Names
Adapter protein GRID; GRB-2-like protein; GRB2L; GRBLG; GRBX; Grf40 adapter protein; Grf-40; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL; P38; Protein GADS; SH3-SH2-SH3 adapter Mona
UniProt Gene Name
GRAP2
UniProt Synonym Gene Names
GADS; GRB2L; GRID; GRB2L; Grf-40
NCBI Protein Information
GRB2-related adapter protein 2

NCBI Summary for GRAP2

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
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UniProt Comments for GRAP2

GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; endosome; nucleoplasm; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: cell-cell signaling; Ras protein signal transduction; T cell costimulation; T cell receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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