Mouse Anti-ESRA; Monoclonal Antibody – Cat# MBS475265

Western blot analysis of extracts from CHO-K1 (B) and CHO-K1 transfected by Estrogen Receptor?alpha fragment (A) cell lysates using Estrogen Receptor?alpha mouse mAb (1:2000 diluted). Predicted band size:50KDa. Observed band size:50KDa.

Estrogen Receptor Alpha (ESRA;) Antibody – Mouse Monoclonal

Name: ESRA; Antibody; Monoclonal; Mouse; Transfected-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS475265
Price: 525 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475265

Entrez Summary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Estrogen Receptor alpha (ESRA;), Monoclonal Antibody

Full Product Name

Anti-Estrogen Receptor alpha Mouse mAb

Product Gene Name

anti-ESRA; antibody

Product Synonym Gene Name

esr1; ER; ESR; Era; ESTRR; NR3A1.

Product Synonym Names

Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER Beta; ER-alpha; ER[a]; ER[b]; Era; ERalpha; Erb; Erb2; ERbeta; ESR; ESR BETA; ESR1; ESR1_HUMAN; ESR2; ESRA; ESRB; Estr; Estra; Estradiol Receptor alpha; Estradiol receptor; Estradiol Receptor beta; ESTRB; Estrogen nuclear receptor alpha; Estrogen receptor 1 (alpha); Estrogen Receptor 1; Estrogen receptor 2 (ER beta); Estrogen Receptor 2; Estrogen receptor 2 ER beta; Estrogen receptor alpha; Estrogen receptor alpha 3*, 4, 5, 6, 7*/822 isoform; Estrogen receptor alpha delta 3*, 4, 5, 6, 7*, 8*/941 isoform; Estrogen receptor alpha delta 3*, 4, 5, 6, 7*/819 2 isoform; Estrogen receptor alpha delta 4 +49 isoform; Estrogen receptor alpha delta 4*, 5, 6, 7*/654 isoform; Estrogen receptor alpha delta 4*, 5, 6, 7, 8*/901 isoform; Estrogen receptor alpha E1 E2 1 2; Estrogen receptor alpha E1 N2 E2 1 2; Estrogen receptor; Estrogen receptor beta 4; Estrogen resistance, included; ESTRR; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; NR3A2; Nuclear receptor subfamily 3 group A member 1; Nuclear receptor subfamily 3 group A member 2; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

6F11-C3-G6

Host

Mouse
Source: Human

Species Reactivity

Transfected

Sensitivity

This antibody detects endogenous levels of Estrogen Receptor alpha and does not cross-react with related proteins.

Form/Format

PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Purity/Purification

Affinity Purified

Concentration

2.85mg/ml (lot specific)

Molecular Weight

OMIM

X03635 mRNA

3D Structure

ModBase 3D Structure for P03372

Immunogen

Purified recombinant human Estrogen Receptor alphaMMP-2 protein expressed in E Coli.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Subunit Structure

Binds DNA as a homodimer. Can form a heterodimer with ESR2. Isoform 3 can probably homodimerize or heterodimerize with isoform 1 and ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (By similarity). Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is a ligand-inducible. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with KMT2D/MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interaction with SH2D4A blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PI3KR1 or PI3KR2, SRC and PTK2/FAK1. Interacts with RBFOX2. Interacts with STK3/MST2 only in the presence of SAV1 and vice-versa. Binds to CSNK1D. Interacts with NCOA2; NCOA2 can interact with ESR1 AF-1 and AF-2 domains simultaneously and mediate their transcriptional synergy. Interacts with DDX5. Interacts with NCOA1; the interaction seems to require a self-association of N-terminal and C-terminal regions. Interacts with ZNF366, DDX17, NFKB1, RELA, SP1 and SP3. Interacts with NRIP1 (By similarity). Interacts with GPER1; the interaction occurs in an estrogen-dependent manner. Interacts with CLOCK and the interaction is stimulated by estrogen. Interacts with BCAS3. Interacts with TRIP4 (ufmylated); estrogen dependent.

Subcellular Location

Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.

Applications Tested/Suitable for anti-ESRA; antibody

Western Blot (WB)

Application Notes for anti-ESRA; antibody

WB: 1:1000

Other Notes

Small volumes of anti-ESRA; antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ESRA; purchase

MBS475265 is an antibody targeting ESRA;. Also known as Anti-Estrogen Receptor alpha Mouse mAb. Reactive in Transfected. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for ESRA;. It may not necessarily be applicable to this product.

NCBI GI #

62821794

NCBI GeneID

2099

NCBI Accession #

NP_000116.2

UniProt Primary Accession #

P03372

UniProt Secondary Accession #

Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7

UniProt Related Accession #

P03372

GenBank Nucleotide #

NM_000125

NCBI Official Full Name

estrogen receptor isoform 1

NCBI Official Synonym Full Names

estrogen receptor 1

NCBI Official Symbol

ESR1

NCBI Official Synonym Symbols

ER; ESR; Era; ESRA; ESTRR; NR3A1

UniProt Protein Name

Estrogen receptor

UniProt Synonym Protein Names

ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

UniProt Gene Name

ESR1

UniProt Synonym Gene Names

ESR; NR3A1; ER

NCBI Protein Information

estrogen receptor

NCBI Summary for ESRA;

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

UniProt Comments for ESRA;

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

Research Articles on ESRA;

1. Human influenza hemagglutinin-tagged PPARGC1B coprecipitated more intensely with ERalpha in the +102525A than the +102525G construct after 17beta estradiol treatment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ESRA; antibody

Reference Product	PubMed Publications
ESR2 antibody	>235 publications with ESRA; and ESR2
JUN antibody	>100 publications with ESRA; and JUN
PGR antibody	>89 publications with ESRA; and PGR
ERBB2 antibody	>45 publications with ESRA; and ERBB2
BRCA1 antibody	>27 publications with ESRA; and BRCA1
MYC antibody	>25 publications with ESRA; and MYC
EGFR antibody	>20 publications with ESRA; and EGFR
CCND1 antibody	>19 publications with ESRA; and CCND1
TP53 antibody	>14 publications with ESRA; and TP53
SP1 antibody	>11 publications with ESRA; and SP1

Pathways associated with anti-ESRA; antibody

Products by Pathway	Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Estrogen Signaling Pathway antibodies	Estrogen Signaling Pathway Diagram
Estrogen Signaling Pathway antibodies	Estrogen Signaling Pathway Diagram
Estrogen Signaling Pathway antibodies	Estrogen Signaling Pathway Diagram
FOXA1 Transcription Factor Network Pathway antibodies	FOXA1 Transcription Factor Network Pathway Diagram
FOXM1 Transcription Factor Network Pathway antibodies	FOXM1 Transcription Factor Network Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Integrated Breast Cancer Pathway antibodies	Integrated Breast Cancer Pathway Diagram

Diseases associated with anti-ESRA; antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>349 publications with ESRA; and Neoplasms
Breast Neoplasms Antibodies	>220 publications with ESRA; and Breast Neoplasms
Female Urogenital Diseases Antibodies	>87 publications with ESRA; and Female Urogenital Diseases
Carcinoma Antibodies	>85 publications with ESRA; and Carcinoma
Adenocarcinoma Antibodies	>43 publications with ESRA; and Adenocarcinoma
Disease Progression Antibodies	>38 publications with ESRA; and Disease Progression
Bone Diseases, Metabolic Antibodies	>35 publications with ESRA; and Bone Diseases, Metabolic
Obesity Antibodies	>32 publications with ESRA; and Obesity
Coronary Artery Disease Antibodies	>23 publications with ESRA; and Coronary Artery Disease
Prostatic Neoplasms Antibodies	>22 publications with ESRA; and Prostatic Neoplasms

Organs/Tissues associated with anti-ESRA; antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>78 publications with ESRA; and Brain
Uterus Antibodies	>60 publications with ESRA; and Uterus
Ovary Antibodies	>52 publications with ESRA; and Ovary
Heart Antibodies	>41 publications with ESRA; and Heart
Testis Antibodies	>38 publications with ESRA; and Testis
Prostate Antibodies	>33 publications with ESRA; and Prostate
Muscle Antibodies	>28 publications with ESRA; and Muscle
Connective Tissue Antibodies	>26 publications with ESRA; and Connective Tissue
Lung Antibodies	>25 publications with ESRA; and Lung
Lymph Node Antibodies	>17 publications with ESRA; and Lymph Node