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MKK3/6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475429

Entrez Summary: This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point… More details

$525 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
MKK3/6, Monoclonal Antibody
Full Product Name
Anti-MKK3/6 Mouse mAb
Product Gene Name
Product Synonym Gene Name
map2k3; MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2; MAP2K3.
Product Synonym Names
AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP kinase kinase 6; map2k3; MAP2K6; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; Mitogen activated protein kinase kinase 3; Mitogen activated protein kinase kinase 6; MKK 3; MKK3; MKK6; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; PRKMK6; Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6); protein kinase, mitogen-activated, kinase 3; SAPK kinase 2; SAPKK 2; SAPKK 3; SAPKK-2; SAPKK2; SAPKK3; Stress activated protein kinase kinase 2; Stress activated protein kinase kinase 3; Stress-activated protein kinase kinase 2
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8A11-G11-G11
Host
Mouse
Source: Human
Species Reactivity
Human, Rat
Sensitivity
This antibody detects endogenous levels of MKK3 and MKK6 and does not cross-react with related proteins.
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
3.2mg/ml (lot specific)
Molecular Weight
40
OMIM
U39657 mRNA
3D Structure
ModBase 3D Structure for P52564
Immunogen
Purified recombinant human MKK6 protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Subunit Structure
MKK3:Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with ARRB1. Interacts with Yersinia yopJ. MKK6: Dimer. Interacts with Yersinia yopJ. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity). Interacts (via its DVD domain) with MAP3Ks activators like MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2 (By similarity). Interacts with DCTN1. Interacts with EIF2AK2/PKR.
Subcellular Location
Cytoplasm ; Nucleus
Applications Tested/Suitable for anti-MKK3/6 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MKK3/6 antibody
WB: 1:1000

Western Blot (WB) of anti-MKK3/6 antibody

Western blot analysis of extracts from PC12, C6, Ramos, Jurkat and Hela cell lysates using MKK3/6 mouse mAb (1:1000 diluted).Predicted band size:40KDa.Observed band size:40KDa.
Mouse Anti-MKK3/6 Monoclonal Antibody – Cat# MBS475429

Immunoprecipitation (IP) of anti-MKK3/6 antibody

Immunoprecipitation analysis of Hela cell lysates using MKK3/6 mouse mAb.
Mouse Anti-MKK3/6 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475429

Other Notes

Small volumes of anti-MKK3/6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MKK3/6 purchase

MBS475429 is an antibody targeting MKK3/6. Also known as Anti-MKK3/6 Mouse mAb. Reactive in Human, Rat. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for MKK3/6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 6 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 6
NCBI Official Symbol
MAP2K6
NCBI Official Synonym Symbols
MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 6
UniProt Synonym Protein Names
MAPK/ERK kinase 6; MEK 6; Stress-activated protein kinase kinase 3; SAPK kinase 3; SAPKK-3; SAPKK3
UniProt Gene Name
MAP2K6
UniProt Synonym Gene Names
MEK6; MKK6; PRKMK6; SKK3; MAP kinase kinase 6; MAPKK 6; MEK 6; SAPK kinase 3; SAPKK-3; SAPKK3
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 6

NCBI Summary for MKK3/6

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]
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UniProt Comments for MKK3/6

MKK6: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. Three alternatively spliced isoforms have been reported.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: MAP kinase kinase activity; protein binding; protein kinase binding; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; cell cycle arrest; DNA damage induced protein phosphorylation; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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