FAK Antibody – Mouse Monoclonal

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015]

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: EC 2.7.10.2; Fak family; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; nucleus; plasma membrane; stress fiber

Molecular Function: actin binding; JUN kinase binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein kinase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; SH2 domain binding

Biological Process: angiogenesis; axon guidance; cell motility involved in cell locomotion; cell structure disassembly during apoptosis; embryonic development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; establishment of cell polarity; heart morphogenesis; innate immune response; integrin-mediated signaling pathway; MAPKKK cascade; negative regulation of apoptosis; negative regulation of cell-cell adhesion; peptidyl-tyrosine phosphorylation; placenta development; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; regulation of cell adhesion mediated by integrin; regulation of cell proliferation; regulation of cell shape; regulation of cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity; regulation of osteoblast differentiation; regulation of protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway