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WNT5B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475540

Entrez Summary: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning… More details

$525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Wnt5b, Monoclonal Antibody
Full Product Name
Anti-Wnt5b Mouse mAb
Product Gene Name
Product Synonym Gene Name
WNT5B
Product Synonym Names
MGC2648; Protein Wnt-5b; Wingless type MMTV integration site family, member 5B; WNT 5B; WNT 5B protein; Wnt5b; WNT5B protein; WNT5B_HUMAN
Clonality
Monoclonal
Isotype
lgG2b
Clone Number
3D1-F4-E9
Host
Mouse
Species Reactivity
Transfected
Sensitivity
Transfected Only.
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
4.8mg/ml (lot specific)
Molecular Weight
45
OMIM
AY009399 mRNA
3D Structure
ModBase 3D Structure for Q9H1J7
Immunogen
Purified recombinant human Wnt5b protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the Wnt family.
Subunit Structure
Interacts with PORCN.
Subcellular Location
Secreted; extracellular space; extracellular matrix
Applications Tested/Suitable for anti-Wnt5b antibody
Western Blot (WB)
Application Notes for anti-Wnt5b antibody
WB: 1:1000

Western Blot (WB) of anti-Wnt5b antibody

Western blot detection of Wnt5b in CHO-K1 cell lysate (B) and CHO-K1 transfected by Wnt5b (A) cell lysate using Wnt5b mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size:45KDa.
Mouse Anti-WNT5B Monoclonal Antibody – Cat# MBS475540

Other Notes

Small volumes of anti-Wnt5b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Wnt5b purchase

MBS475540 is an antibody targeting Wnt5b. Also known as Anti-Wnt5b Mouse mAb. Reactive in Transfected. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Wnt5b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein Wnt-5b
NCBI Official Synonym Full Names
Wnt family member 5B
NCBI Official Symbol
WNT5B
UniProt Protein Name
Protein Wnt-5b
UniProt Gene Name
WNT5B
NCBI Protein Information
protein Wnt-5b

NCBI Summary for Wnt5b

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
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UniProt Comments for Wnt5b

WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.33

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane

Molecular Function: frizzled binding; receptor binding

Biological Process: cell fate commitment; chondrocyte differentiation; neuron differentiation; positive regulation of cell migration; Wnt receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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