GST Antibody – Rabbit Polyclonal

Polyclonal

IgG

Rabbit
Source: Schistosoma japonicum

GST tag from pGEX plasmid

This antibody detects recombinant GST and GST fused proteins and does not cross-react with related proteins.

In PBS (pH 7.4) containing with 0.1mg/ml BSA and 50% glycerol.

Affinity Purified

0.3mg/ml (lot specific)

26

Purified recombinant Escherichia coli BL21 (DE3) GST protein expressed in E Coli.

Protein sequence and structure are important classification methods for GSTs:while classes from the cytosolic superfamily of GSTs possess more than 40% sequence homology, those from other classes may have less than 25%. Cytosolic GSTs are divided into 13 classes based upon their structure:alpha, beta, delta, epsilon, zeta, theta, mu, nu, pi, sigma, tau, phi, and omega.Mitochondrial GSTs are in class kappa.The MAPEG superfamily of microsomal GSTs consists of subgroups designated I-IV, between which amino acid sequences share less than 20% identity.The glutathione binding site, or "G-site, " is located in the thioredoxin-like domain of both cytosolic and mitochondrial GSTs.The region containing the greatest amount of variability between the assorted classes is that of helix alpha2, where one of three different amino acid residues interacts with the glycine residue of glutathione.Two subgroups of cytosolic GSTs have been characterized based upon their interaction with glutathione:the Y-GST group, which uses a tyrosine residue to activate glutathione, and the S/C-GST, which instead use serine or cysteine residues.The porcine pi-class enzyme pGTSP1-1 was the first GST to have its structure determined, and it is representative of other members of the cytosolic GST superfamily, which contain a thioredoxin-like N-terminus domain as well as a C-terminus domain consisting of alpha helices.Mammalian cytosolic GSTs are dimeric, both subunits being from the same class of GSTs, although not necessarily identical.The monomers are in the range of 22-30 kDa.They are active over a wide variety of substrates with considerable overlap.

Metabolism; Pathways and Processes; Mitochondrial Metabolism; Mitochondrial markers; Tags & Cell Markers; Fusion/Marker Proteins; GST