Rabbit Anti-PKM2 Polyclonal Antibody – Cat# MBS475566

Western blot detection of PKM2 in COS7, C6, 3T3 and Hela cell lysates using PKM2 Rabbit pAb (1:1000 diluted).Predicted band size:60kDa.Observed band size:60kDa.

PKM2 Antibody – Rabbit Polyclonal

Name: PKM2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS475566
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475566

Entrez Summary: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PKM2, Polyclonal Antibody

Full Product Name

Anti-PKM2 Rabbit pAb

Product Gene Name

anti-PKM2 antibody

Product Synonym Gene Name

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Product Synonym Names

CTHBP; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM_HUMAN; MGC3932; OIP 3; OIP-3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK muscle type; PK, muscle type; PK2; PK3; PKM; PKM2; pykm; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; pyruvate kinase PKM; Pyruvate kinase, muscle 2; TCB; THBP1; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone-binding protein 1; Tumor M2 PK; Tumor M2-PK

Clonality

Polyclonal

Isotype

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Form/Format

10mM PBS with 0.02% sodium azide, 50% glycerol.

Purity/Purification

Affinity Purified

Molecular Weight

OMIM

M23725 mRNA

3D Structure

ModBase 3D Structure for P14618

Immunogen

CVTEVENGGSLGSKKGVNLP

Sequence Similarities

Belongs to the pyruvate kinase family.

Subunit Structure

Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia.

Subcellular Location

Cytoplasm ; Nucleus

Applications Tested/Suitable for anti-PKM2 antibody

Western Blot (WB)

Application Notes for anti-PKM2 antibody

WB: 1:1000

Other Notes

Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM2 purchase

MBS475566 is an antibody targeting PKM2. Also known as Anti-PKM2 Rabbit pAb. Reactive in Human, Mouse, Rat, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.

NCBI GI #

332164775

NCBI GeneID

5315

NCBI Accession #

NP_001193725.1

UniProt Primary Accession #

P14618

UniProt Secondary Accession #

P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6

UniProt Related Accession #

P14618

GenBank Nucleotide #

NM_001206796

NCBI Official Full Name

pyruvate kinase PKM isoform c

NCBI Official Synonym Full Names

pyruvate kinase, muscle

NCBI Official Symbol

PKM

NCBI Official Synonym Symbols

PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

UniProt Protein Name

Pyruvate kinase PKM

UniProt Synonym Protein Names

Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58

UniProt Gene Name

PKM

UniProt Synonym Gene Names

OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1

NCBI Protein Information

pyruvate kinase PKM

NCBI Summary for PKM2

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

UniProt Comments for PKM2

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Research Articles on PKM2

1. PKM2 was involved in progression of ESCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PKM2 antibody

Products by Pathway	Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway antibodies	Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with anti-PKM2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>21 publications with PKM2 and Neoplasms
Drug Toxicity Antibodies	>9 publications with PKM2 and Drug Toxicity
Lung Diseases Antibodies	>8 publications with PKM2 and Lung Diseases
Fibrosis Antibodies	>8 publications with PKM2 and Fibrosis
Nervous System Diseases Antibodies	>8 publications with PKM2 and Nervous System Diseases
Inflammation Antibodies	>6 publications with PKM2 and Inflammation
Cardiovascular Diseases Antibodies	>6 publications with PKM2 and Cardiovascular Diseases
Cell Transformation, Neoplastic Antibodies	>5 publications with PKM2 and Cell Transformation, Neoplastic
Liver Diseases Antibodies	>4 publications with PKM2 and Liver Diseases
Necrosis Antibodies	>4 publications with PKM2 and Necrosis

Organs/Tissues associated with anti-PKM2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>49 publications with PKM2 and Brain
Blood Antibodies	>35 publications with PKM2 and Blood
Muscle Antibodies	>20 publications with PKM2 and Muscle
Kidney Antibodies	>14 publications with PKM2 and Kidney
Liver Antibodies	>13 publications with PKM2 and Liver
Heart Antibodies	>10 publications with PKM2 and Heart
Nerve Antibodies	>8 publications with PKM2 and Nerve
Vascular Antibodies	>8 publications with PKM2 and Vascular
Lung Antibodies	>7 publications with PKM2 and Lung
Intestine Antibodies	>4 publications with PKM2 and Intestine