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GAD2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475590

Entrez Summary: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic… More details

$525 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAD2, Monoclonal Antibody
Full Product Name
Anti-GAD2 Mouse mAb
Product Gene Name
Product Synonym Gene Name
GAD65
Product Synonym Names
65 kDa glutamic acid decarboxylase; DCE 2; DCE2; DCE2_HUMAN; GAD 2; GAD 65; GAD-2; GAD-65; Gad2; Glutamate decarboxylase 2 (pancreas); Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa); Glutamate decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65; MGC161605; MGC161607
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6D5-E8-E4
Host
Mouse
Species Reactivity
Human, Rat
Sensitivity
This antibody detects endogenous levels of GAD2 and does not cross-react with related proteins.
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
4.76mg/ml (lot specific)
Molecular Weight
60kDa
OMIM
M81882 mRNA
3D Structure
ModBase 3D Structure for Q05329
Immunogen
Purified recombinant human GAD2 protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the group II decarboxylase family.
Subunit Structure
Homodimer.
Subcellular Location
Cytoplasm; cytosol. Cytoplasmic vesicle. Cell junction; synapse; presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
Applications Tested/Suitable for anti-GAD2 antibody
Western Blot (WB)
Application Notes for anti-GAD2 antibody
WB: 1:1000

Western Blot (WB) of anti-GAD2 antibody

Western blot detection of GAD2 in Rat Hippocampus and Rat Brain lysates using GAD2 mouse mAb (dilution 1:1000).Predicted band size:60kDa.Observed band size:60kDa.
Mouse Anti-GAD2 Monoclonal Antibody – Cat# MBS475590

Other Notes

Small volumes of anti-GAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD2 purchase

MBS475590 is an antibody targeting GAD2. Also known as Anti-GAD2 Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2
NCBI Official Symbol
GAD2
NCBI Official Synonym Symbols
GAD65
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Synonym Protein Names
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
UniProt Gene Name
GAD2
UniProt Synonym Gene Names
GAD65; GAD-65
NCBI Protein Information
glutamate decarboxylase 2

NCBI Summary for GAD2

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
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UniProt Comments for GAD2

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: plasma membrane

Molecular Function: glutamate decarboxylase activity; protein binding

Biological Process: neurotransmitter secretion; synaptic transmission
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-GAD2 antibody

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