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NEDD8 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475624

MBS475624 is an antibody targeting NEDD8. Also known as Anti-NEDD8 Mouse mAb. Reactive in Human, Rat. Host: MouseSource: Human. More details

$525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEDD8, Monoclonal Antibody
Full Product Name
Anti-NEDD8 Mouse mAb
Product Gene Name
Product Synonym Gene Name
NEDD-8
Product Synonym Names
FLJ43224; MGC104393; MGC125896; MGC125897; NED8; NEDD 8; NEDD-8; Nedd8; NEDD8_HUMAN; Neddylin; Neural precursor cell expressed developmentally own regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Neural precursor cell expressed developmentally down-regulated protein 8; Rub1; Ubiquitin like protein Nedd 8; Ubiquitin like protein Nedd8; Ubiquitin-like protein Nedd8
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5B8-D7-C5
Host
Mouse
Source: Human
Species Reactivity
Human, Rat
Sensitivity
The antibody detects endogenous level of total NEDD8 protein.
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Purity/Purification
Affinity Purified
Concentration
1mg/ml (lot specific)
Molecular Weight
90
OMIM
D23662 mRNA
3D Structure
ModBase 3D Structure for Q15843
Immunogen
Purified recombinant fragment of human NEDD8 expressed in E Coli.
Sequence Similarities
Belongs to the ubiquitin family.
Subunit Structure
Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.
Subcellular Location
Nucleus. Note: Mainly nuclear.
Applications Tested/Suitable for anti-NEDD8 antibody
Western Blot (WB)
Application Notes for anti-NEDD8 antibody
WB: 1:500

Western Blot (WB) of anti-NEDD8 antibody

Western blot detection of NEDD8 in C6 and Hela cell lysates using NEDD8 mouse mAb (dilution 1:500).Predicted band size:9KDa.Observed band size:90KDa.
Mouse Anti-NEDD8 Monoclonal Antibody – Cat# MBS475624

Other Notes

Small volumes of anti-NEDD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEDD8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
UniProt Protein Name
NEDD8
UniProt Synonym Protein Names
Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Protein Family
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
NCBI Protein Information
NEDD8

UniProt Comments for NEDD8

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: anatomical structure morphogenesis; protein modification process; protein neddylation; proteolysis; transforming growth factor beta receptor signaling pathway; ubiquitin-dependent protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEDD8 antibody

 Products by Pathway  Pathway Diagram
 P53 Pathway antibodies  P53 Pathway Diagram
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