BRK Antibody – Mouse Monoclonal

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Brk: a tyrosine kinase of the Src family. May function as an intracellular signal transducer in epithelial tissues. Very high level expression in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Selectively expressed in breast tumors and cell lines, and perhaps in colon and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression in mammary cells leads to EGF sensitization and results in a partially transformed phenotype. Enhances anchorage-independent growth and responsiveness to EGF. RNAi reduces proliferation in breast cancer cells. Kinase-inactive mutant indicates tumor function may be independent of catalytic function.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; nucleoplasm; nucleus; ruffle

Molecular Function: identical protein binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: cell migration; innate immune response; negative regulation of signal transduction; positive regulation of cell cycle; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein