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Keratin 8 (KRT8) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475863

Entrez Summary: This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene… More details

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Product Overview

Product Name
Keratin 8 (KRT8), Monoclonal Antibody
Full Product Name
Anti-Keratin 8 Mouse mAb
Product Gene Name
Product Synonym Gene Name
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Product Synonym Names
CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2F4-H9-D2
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Molecular Weight
55kDa
OMIM
M34482 Genomic DNA
3D Structure
ModBase 3D Structure for P05787
Immunogen
Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E Coli.
Sequence Similarities
Belongs to the intermediate filament family.
Subunit Structure
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Interacts with GPER1.
Subcellular Location
Nucleus matrix
Applications Tested/Suitable for anti-KRT8 antibody
Western Blot (WB)

Western Blot (WB) of anti-KRT8 antibody

Western blot detection of Keratin 8 in HepG2, Hela, MCF7 and A549 cell lysates using Keratin 8 mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.
Mouse Anti-KRT8 Monoclonal Antibody – Cat# MBS475863

Immunofluorescence (IF) of anti-KRT8 antibody

Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 mouse mAb (green), showing cytoplasmic localization. Blue
Mouse Anti-KRT8 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS475863

Immunohistochemistry (IHC) of anti-KRT8 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb.
Mouse Anti-KRT8 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS475863

Immunohistochemistry (IHC) of anti-KRT8 antibody

Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb
Mouse Anti-KRT8 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS475863

Other Notes

Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT8 purchase

MBS475863 is an antibody targeting KRT8. Also known as Anti-Keratin 8 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type II cytoskeletal 8 isoform 2
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Synonym Protein Names
Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Protein Family
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8; CK-8; K8
NCBI Protein Information
keratin, type II cytoskeletal 8

NCBI Summary for KRT8

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for KRT8

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoplasm; intermediate filament; nucleus

Molecular Function: protein binding

Disease: Cirrhosis, Familial
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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