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ESET Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476097

Entrez Summary: This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N (6)-methyl-L-lysine. The encoded protein likely… More details

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Product Overview

Online Datasheet in PDF
Product Name
ESET, Monoclonal Antibody
Full Product Name
Anti-ESET Mouse mAb
Product Gene Name
anti-ESET antibody
Product Synonym Gene Name
SETDB1; ESET; KG1T; KMT1E; KIAA0067; H3-K9-HMTase4; SETDB1
Product Synonym Names
ESET; KG1T; KMT1E; KIAA0067; H3-K9-HMTase4; SETDB1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1E7-F3-C6
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Molecular Weight
180kDa
OMIM
D31891 mRNA
3D Structure
ModBase 3D Structure for Q15047
Immunogen
Purified recombinant fragment of human SETDB1 expressed in E Coli.
Sequence Similarities
Contains 2 Tudor domains.
Subunit Structure
Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 (PubMed:19061646). During DNA replication, it is recruited by SETDB1 to form a S phase-specific complex that facilitates methylation of H3 Lys-9 during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1 (PubMed:15327775). Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1 (PubMed:24623306). Interacts with TRIM28/TIF1B (PubMed:11959841). Interacts with ATF7IP and ATF7IP2; the interaction with ATF7IP is required to stimulate histone methyltransferase activity and facilitate the conversion of dimethylated to trimethylated H3 Lys-9 (PubMed:14536086, PubMed:15691849). Interacts with MBD1; interaction is abolished when MBD1 is sumoylated (PubMed:15327775, PubMed:17066076). Interacts with CBX1 and CBX5 (PubMed:15899859). Interacts with DNMT3A and DNMT3B (PubMed:16682412). Interacts with SUMO2. Interacts with CHD7, NLK1 and PPARG (PubMed:17952062). Interacts with MPHOSPH8 (PubMed:20871592). Interacts with ERG (By similarity). Interacts with HDAC1, HDAC2, SIN3A and SIN3B (By similarity). Interacts with ATRX. Forms a complex with ATRX, TRIM28 and ZNF274 (PubMed:27029610).
Subcellular Location
Chromosome
Applications Tested/Suitable for anti-ESET antibody
Western Blot (WB)

Western Blot (WB) of anti-ESET antibody

Western blot detection of ESET in Jurkat, A549, MCF7 and C6 cell lysates using ESET mouse mAb (1:2000 diluted).Predicted band size:180KDa.Observed band size:180KDa.
Mouse Anti-ESET Monoclonal Antibody – Cat# MBS476097

Immunofluorescence (IF) of anti-ESET antibody

Immunofluorescence analysis of LOVO cells using SETDB1 mouse mAb (green). Red
Mouse Anti-ESET Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS476097

Other Notes

Small volumes of anti-ESET antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ESET purchase

MBS476097 is an antibody targeting ESET. Also known as Anti-ESET Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ESET. It may not necessarily be applicable to this product.

NCBI GI #
224177467
NCBI GeneID
9869
NCBI Accession #
NP_001138887.1
UniProt Primary Accession #
Q15047
UniProt Secondary Accession #
Q5SZD8; Q5SZD9; Q5SZE0; Q5SZE7; Q96GM9; A6NEW2
UniProt Related Accession #
Q15047
GenBank Nucleotide #
NM_001145415.1
NCBI Official Full Name
histone-lysine N-methyltransferase SETDB1 isoform 1
NCBI Official Synonym Full Names
SET domain bifurcated 1
NCBI Official Symbol
SETDB1
NCBI Official Synonym Symbols
ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
UniProt Protein Name
Histone-lysine N-methyltransferase SETDB1
UniProt Synonym Protein Names
ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
SETDB1
UniProt Synonym Gene Names
KIAA0067; KMT1E; ESET; H3-K9-HMTase 4
NCBI Protein Information
histone-lysine N-methyltransferase SETDB1

NCBI Summary for ESET

This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]
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UniProt Comments for ESET

SETDB1: a protein lysine methyltransferase that specifically trimethylates K9 of histone H3 (H3K9me3), a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Unlike SUV39H H3K9 methyltransferase, which functions mainly in heterochromatin regions such as pericentric heterochromatin, SETDB1 functions mainly in euchromatic regions, playing a central role in the silencing of euchromatic genes. H3K9me3 is coordinated with DNA methylation. Interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. Contains Tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. Is targeted to histone H3 by TIF1B, a factor recruited by KRAB zinc-finger proteins. Recruited by DNMT3A to silenced promoters in cancer cells. May play a role in the pathogenesis of Huntington's disease, since levels of SETDB1 and H3K9me3 are both increased in diseased brains. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleoplasm; nucleus; plasma membrane

Molecular Function: chromatin binding; protein binding

Biological Process: Ras protein signal transduction
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Research Articles on ESET

1. These results suggest that SETDB1- mediated FosB expression is a common molecular phenomenon, and might be a novel pathway responsible for the increase in cell proliferation that frequently occurs during anticancer drug therapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ESET antibody

 Reference Product  PubMed Publications
 PPARG antibody  >8 publications with ESET and PPARG
 DNMT3A antibody  >7 publications with ESET and DNMT3A
 MBD1 antibody  >6 publications with ESET and MBD1
 ATF7IP antibody  >5 publications with ESET and ATF7IP
 SUMO2 antibody  >1 publications with ESET and SUMO2

Pathways associated with anti-ESET antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Noncanonical Wnt Signaling Pathway antibodies  Noncanonical Wnt Signaling Pathway Diagram
 PKMTs Methylate Histone Lysines Pathway antibodies  PKMTs Methylate Histone Lysines Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram

Diseases associated with anti-ESET antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >7 publications with ESET and Carcinoma
 Melanoma Antibodies  >5 publications with ESET and Melanoma
 Lung Neoplasms Antibodies  >4 publications with ESET and Lung Neoplasms
 Skin Neoplasms Antibodies  >2 publications with ESET and Skin Neoplasms
 Hypertrophy Antibodies  >2 publications with ESET and Hypertrophy
 Nervous System Diseases Antibodies  >2 publications with ESET and Nervous System Diseases
 Inflammation Antibodies  >2 publications with ESET and Inflammation
 Prostatic Diseases Antibodies  >1 publications with ESET and Prostatic Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with ESET and Cell Transformation, Neoplastic
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with ESET and Prenatal Exposure Delayed Effects

Organs/Tissues associated with anti-ESET antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >11 publications with ESET and Bone
 Brain Antibodies  >10 publications with ESET and Brain
 Embryonic Tissue Antibodies  >8 publications with ESET and Embryonic Tissue
 Lung Antibodies  >7 publications with ESET and Lung
 Connective Tissue Antibodies  >4 publications with ESET and Connective Tissue
 Blood Antibodies  >4 publications with ESET and Blood
 Skin Antibodies  >3 publications with ESET and Skin
 Eye Antibodies  >3 publications with ESET and Eye
 Muscle Antibodies  >3 publications with ESET and Muscle
 Bone Marrow Antibodies  >3 publications with ESET and Bone Marrow
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