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BBC3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4761880

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. More details

$525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PUMA (BBC3), Monoclonal Antibody
Full Product Name
PUMA Rabbit mAb
Product Gene Name
anti-BBC3 antibody
Product Synonym Gene Name
PUMA
Product Synonym Names
BBC3; PUMA; Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis
Clonality
Monoclonal
Isotype
IgG
Clone Number
R05-9C7
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Purity/Purification
Affinity Purified
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
Molecular Weight
193
OMIM
605854
3D Structure
ModBase 3D Structure for Q9BXH1
Immunogen
A synthetic peptide of human PUMA
Conjugation
Unconjugated
Modification
Unmodified
Research Area
Cell Biology

Product Categories/Family for anti-BBC3 antibody

Home; Products; Featured Collections; Recombinant Rabbit Monoclonal Antibodies; Primary antibody
Applications Tested/Suitable for anti-BBC3 antibody
Western Blot (WB), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-BBC3 antibody
WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200

Immunocytochemistry (ICC) of anti-BBC3 antibody

Immunocytochemistry analysis of PUMA (green) in 293T using PUMA antibody,and DAPI(blue).
Rabbit Anti-BBC3 Monoclonal Antibody – Cat# MBS4761880

Western Blot (WB) of anti-BBC3 antibody

Western blot analysis of PUMA in K562 lysates using PUMA antibody.
Rabbit Anti-BBC3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4761880

Western Blot (WB) of anti-BBC3 antibody

Western blot analysis of PUMA in Jurkat, THP-1, C6, 3T3, Hela lysates using PUMA antibody
Rabbit Anti-BBC3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4761880

Immunohistochemistry (IHC) of anti-BBC3 antibody

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PUMA antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Rabbit Anti-BBC3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4761880

Other Notes

Small volumes of anti-BBC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BBC3 purchase

MBS4761880 is an antibody targeting BBC3. Also known as PUMA Rabbit mAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BBC3. It may not necessarily be applicable to this product.

NCBI GI #
56748610
NCBI GeneID
27113
NCBI Accession #
Q9BXH1.1
UniProt Primary Accession #
Q9BXH1
UniProt Secondary Accession #
O00171; Q96PG9; B9EGI3
UniProt Related Accession #
Q96PG8; Q9BXH1
NCBI Official Full Name
Bcl-2-binding component 3
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
BBC3
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
UniProt Protein Name
Bcl-2-binding component 3
UniProt Synonym Protein Names
JFY-1; p53 up-regulated modulator of apoptosis
Protein Family
Bcl-2-binding component
UniProt Gene Name
BBC3
UniProt Synonym Gene Names
PUMA
UniProt Entry Name
BBC3_HUMAN
NCBI Protein Information
bcl-2-binding component 3; p53 up-regulated modulator of apoptosis

NCBI Summary for BBC3

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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UniProt Comments for BBC3

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
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Research Articles on BBC3

1. c-Jun transactivates Puma gene expression to promote osteoarthritis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BBC3 antibody

 Reference Product  PubMed Publications
 PMAIP1 antibody  >72 publications with BBC3 and PMAIP1
 TP53 antibody  >59 publications with BBC3 and TP53
 BCL2L1 antibody  >46 publications with BBC3 and BCL2L1
 BID antibody  >26 publications with BBC3 and BID
 BAD antibody  >24 publications with BBC3 and BAD
 MCL1 antibody  >17 publications with BBC3 and MCL1
 SP1 antibody  >3 publications with BBC3 and SP1
 SNAI2 antibody  >3 publications with BBC3 and SNAI2
 BCL2L2 antibody  >3 publications with BBC3 and BCL2L2

Pathways associated with anti-BBC3 antibody

 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies  BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-BBC3 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >73 publications with BBC3 and Carcinoma
 Adenocarcinoma Antibodies  >33 publications with BBC3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >32 publications with BBC3 and Breast Neoplasms
 Lung Neoplasms Antibodies  >24 publications with BBC3 and Lung Neoplasms
 Necrosis Antibodies  >20 publications with BBC3 and Necrosis
 Liver Diseases Antibodies  >18 publications with BBC3 and Liver Diseases
 Lung Diseases Antibodies  >17 publications with BBC3 and Lung Diseases
 Nervous System Diseases Antibodies  >16 publications with BBC3 and Nervous System Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with BBC3 and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >13 publications with BBC3 and Brain Diseases

Organs/Tissues associated with anti-BBC3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >30 publications with BBC3 and Lung
 Brain Antibodies  >18 publications with BBC3 and Brain
 Kidney Antibodies  >13 publications with BBC3 and Kidney
 Skin Antibodies  >12 publications with BBC3 and Skin
 Prostate Antibodies  >12 publications with BBC3 and Prostate
 Stomach Antibodies  >12 publications with BBC3 and Stomach
 Muscle Antibodies  >9 publications with BBC3 and Muscle
 Bone Antibodies  >9 publications with BBC3 and Bone
 Eye Antibodies  >7 publications with BBC3 and Eye
 Intestine Antibodies  >6 publications with BBC3 and Intestine
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