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PINCH Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476192

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal… More details

$525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PINCH, Monoclonal Antibody
Full Product Name
Anti-PINCH
Product Gene Name
Product Synonym Gene Name
LIMS1; PINCH; PINCH1; PINCH-1
Product Synonym Names
2310016J22Rik; 4921524A02Rik; AI507642; AU021743; AW551584; C430041B13Rik; LIM and senescent cell antigen like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIM zinc finger domain containing 1; LIM-type zinc finger domains 1; Lims1; LIMS1_HUMAN; Lims1l; Particularly interesting new Cys His protein 1; Particularly interesting new Cys His protein; Particularly interesting new Cys-His protein 1; PINCH 1; PINCH; PINCH-1; PINCH1; Renal carcinoma antigen NY REN 48; Renal carcinoma antigen NY-REN-48; senescent cell antigen; wu:fc32c03; zgc:112533
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C12-F7-A8
Host
Mouse
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin300, pH 7.3
Purity/Purification
Ascitic Fluid
Preparation and Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycle.
Molecular Weight
37kDa
OMIM
U09284 mRNA
3D Structure
ModBase 3D Structure for P48059
Antibody Type
Primary antibody
Immunogen
Purified recombinant fragment of human PINCH expressed in E Coli.
Cross Reactivity
Human
Subcellular Location
Cell junction; Cell membrane; Membrane
Applications Tested/Suitable for anti-PINCH antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC), Immunocytochemistry (ICC)/Immunofluorescence (IF)
Application Notes for anti-PINCH antibody
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400

Western Blot (WB) of anti-PINCH antibody

Western blot detection of PINCH in C6, Jurkat, K562, 3T3 and Hela cell lysates using PINCH mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:35KDa.
Mouse Anti-PINCH Monoclonal Antibody – Cat# MBS476192

Western Blot (WB) of anti-PINCH antibody

Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
Mouse Anti-PINCH Monoclonal Antibody, Western Blot (WB) – Cat# MBS476192

Immunoprecipitation (IP) of anti-PINCH antibody

Immunoprecipitation analysis of Hela cell lysates using PINCH mouse mAb.
Mouse Anti-PINCH Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS476192

Immunofluorescence (IF) of anti-PINCH antibody

Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue
Mouse Anti-PINCH Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS476192

Flow Cytometry (FC/FACS) of anti-PINCH antibody

Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
Mouse Anti-PINCH Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476192

Other Notes

Small volumes of anti-PINCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PINCH purchase

MBS476192 is an antibody targeting PINCH. Also known as Anti-PINCH. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PINCH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
LIM and senescent cell antigen-like-containing domain protein 1 isoform c
NCBI Official Synonym Full Names
LIM zinc finger domain containing 1
NCBI Official Symbol
LIMS1
NCBI Official Synonym Symbols
PINCH; PINCH1; PINCH-1
UniProt Protein Name
LIM and senescent cell antigen-like-containing domain protein 1
UniProt Synonym Protein Names
Particularly interesting new Cys-His protein 1; PINCH-1; Renal carcinoma antigen NY-REN-48
UniProt Gene Name
LIMS1
UniProt Synonym Gene Names
PINCH; PINCH1; PINCH-1
NCBI Protein Information
LIM and senescent cell antigen-like-containing domain protein 1

UniProt Comments for PINCH

Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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