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Dimethyl-Histone H3 (H3-4) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4762198

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core… More details

$525 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DiMethyl-Histone H3 (H3-4), Monoclonal Antibody
Full Product Name
DiMethyl-Histone H3 (Lys4) Rabbit mAb
Product Gene Name
Product Synonym Names
H3K4me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Clonality
Monoclonal
Isotype
IgG
Clone Number
R02-8D7
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Purified
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
Molecular Weight
136
Chromosome Location
Chromosome: 1; NC_000001.10 (228612546..228613026, complement). Location: 1q42
OMIM
602820
3D Structure
ModBase 3D Structure for Q16695
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Conjugation
Unconjugated
Modification
Methylated
Research Area
Epigenetics and Nuclear Signaling
Applications Tested/Suitable for anti-H3-4 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ChImmunoprecipitation (IP)
Application Notes for anti-H3-4 antibody
WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/50
ChIP: 1/20

Western Blot (WB) of anti-H3-4 antibody

Western blot analysis of DiMethyl-Histone H3 (Lys4) in various cell lines lysates using DiMethyl-Histone H3 (Lys4) antibody.
Rabbit Anti-H3-4 Monoclonal Antibody – Cat# MBS4762198

Dot Blot (DB) of anti-H3-4 antibody

DiMethyl-Histone H3-K4 pAb Dotblot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody.
Rabbit Anti-H3-4 Monoclonal Antibody, Dot Blot (DB) – Cat# MBS4762198

Immunohistochemistry (IHC) of anti-H3-4 antibody

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using DiMethyl-Histone H3-K4 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Rabbit Anti-H3-4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4762198

Immunohistochemistry (IHC) of anti-H3-4 antibody

Immunohistochemistry analysis of paraffin-embedded mouse lung using DiMethyl-Histone H3-K4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Rabbit Anti-H3-4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4762198

Other Notes

Small volumes of anti-H3-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3-4 purchase

MBS4762198 is an antibody targeting H3-4. Also known as DiMethyl-Histone H3 (Lys4) Rabbit mAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H3-4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
UniProt Protein Name
Histone H3.1t
UniProt Synonym Protein Names
H3/g
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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