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COTL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476258

Entrez Summary: This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although… More details

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Product Overview

Online Datasheet in PDF
Product Name
COTL1, Monoclonal Antibody
Full Product Name
Anti-COTL1 Mouse mAb
Product Gene Name
Product Synonym Gene Name
CLP
Product Synonym Names
CLP; Coactosin like 1; Coactosin-like protein; COTL1; COTL1_HUMAN; FLJ43657; MGC19733
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4E11-H10-D5
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Purified antibody in PBS with 0.05% sodium azide
Molecular Weight
16kDa
OMIM
L54057 mRNA
3D Structure
ModBase 3D Structure for Q14019
Immunogen
Purified recombinant fragment of human COTL1 expressed in E Coli.
Sequence Similarities
Contains 1 ADF-H domain.
Subunit Structure
Interacts with 5-lipoxygenase (ALOX5/5LO) in a calcium-independent manner. Binds to F-actin with a stoichiometry of 1:2.
Subcellular Location
Nucleus
Applications Tested/Suitable for anti-COTL1 antibody
Western Blot (WB)

Western Blot (WB) of anti-COTL1 antibody

Western blot detection of COTL1 in MCF7 and C6 cell lysates using COTL1 mouse mAb (1:1000 diluted).Predicted band size:16KDa.Observed band size:16KDa.
Mouse Anti-COTL1 Monoclonal Antibody – Cat# MBS476258

Western Blot (WB) of anti-COTL1 antibody

Western blot analysis using COTL1 mAb against HEK293 (1) and COTL1 (AA
Mouse Anti-COTL1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476258

Immunohistochemistry (IHC) of anti-COTL1 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using COTL1 mouse mAb with DAB staining.
Mouse Anti-COTL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476258

Immunohistochemistry (IHC) of anti-COTL1 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using COTL1 mouse mAb with DAB staining.
Mouse Anti-COTL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476258

Other Notes

Small volumes of anti-COTL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COTL1 purchase

MBS476258 is an antibody targeting COTL1. Also known as Anti-COTL1 Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for COTL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
coactosin-like protein
NCBI Official Synonym Full Names
coactosin like F-actin binding protein 1
NCBI Official Symbol
COTL1
NCBI Official Synonym Symbols
CLP
UniProt Protein Name
Coactosin-like protein
UniProt Gene Name
COTL1
UniProt Synonym Gene Names
CLP
NCBI Protein Information
coactosin-like protein

NCBI Summary for COTL1

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008]
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UniProt Comments for COTL1

COTL1: Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Belongs to the actin-binding proteins ADF family. Coactosin subfamily.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 16q24.1

Molecular Function: actin binding; enzyme binding; protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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