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PBK/TOPK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476267

Entrez Summary: This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be… More details

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Product Overview

Online Datasheet in PDF
Product Name
PBK/TOPK, Monoclonal Antibody
Full Product Name
Anti-PBK/TOPK Mouse mAb
Product Gene Name
anti-PBK/TOPK antibody
Product Synonym Gene Name
PBK; SPK; CT84; TOPK; Nori-3
Product Synonym Names
Cancer/testis antigen 84; CT84; Epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine activated killer T cell originated protein kinase; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK like protein kinase; MAPKK-like protein kinase; Nori 3; Nori-3; Nori3; PBK; PDZ binding kinase; PDZ-binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; Spermatogenesis-related protein kinase; SPK; T LAK cell originated protein kinase; T-LAK cell-originated protein kinase; TOPK; TOPK_HUMAN
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4F8-C6-A10
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Molecular Weight
40kDa
OMIM
AB027249 mRNA
3D Structure
ModBase 3D Structure for Q96KB5
Immunogen
Purified recombinant fragment of human PBK expressed in E Coli.
Sequence Similarities
Contains 1 protein kinase domain.
Subunit Structure
Interacts with DLG1 and TP53.
Applications Tested/Suitable for anti-PBK/TOPK antibody
Western Blot (WB)

Western Blot (WB) of anti-PBK/TOPK antibody

Western blot detection of PBK/TOPK in Jurkat, A549, MCF7, C6 and Hela cell lysates using PBK/TOPK mouse mAb (1:5000 diluted).Predicted band size:36KDa.Observed band size:40KDa.
Mouse Anti-PBK/TOPK Monoclonal Antibody – Cat# MBS476267

Western Blot (WB) of anti-PBK/TOPK antibody

Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
Mouse Anti-PBK/TOPK Monoclonal Antibody, Western Blot (WB) – Cat# MBS476267

Immunofluorescence (IF) of anti-PBK/TOPK antibody

Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue
Mouse Anti-PBK/TOPK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS476267

Flow Cytometry (FC/FACS) of anti-PBK/TOPK antibody

Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
Mouse Anti-PBK/TOPK Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476267

Immunohistochemistry (IHC) of anti-PBK/TOPK antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.
Mouse Anti-PBK/TOPK Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476267

Immunohistochemistry (IHC) of anti-PBK/TOPK antibody

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.
Mouse Anti-PBK/TOPK Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476267

Other Notes

Small volumes of anti-PBK/TOPK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PBK/TOPK purchase

MBS476267 is an antibody targeting PBK/TOPK. Also known as Anti-PBK/TOPK Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PBK/TOPK. It may not necessarily be applicable to this product.

NCBI GI #
525507092
NCBI GeneID
55872
NCBI Accession #
NP_001265874.1
UniProt Primary Accession #
Q96KB5
UniProt Secondary Accession #
Q9NPD9; Q9NYL7; Q9NZK6; B4DX68; D3DST2
UniProt Related Accession #
Q96KB5
GenBank Nucleotide #
NM_001278945.1
NCBI Official Full Name
lymphokine-activated killer T-cell-originated protein kinase isoform 2
NCBI Official Synonym Full Names
PDZ binding kinase
NCBI Official Symbol
PBK
NCBI Official Synonym Symbols
SPK; CT84; TOPK; HEL164; Nori-3
UniProt Protein Name
Lymphokine-activated killer T-cell-originated protein kinase
UniProt Synonym Protein Names
Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase
UniProt Gene Name
PBK
UniProt Synonym Gene Names
TOPK; CT84; SPK
NCBI Protein Information
lymphokine-activated killer T-cell-originated protein kinase

NCBI Summary for PBK/TOPK

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for PBK/TOPK

PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein type: Cancer Testis Antigen (CTA); EC 2.7.12.2; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); TOPK family

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: nucleus

Molecular Function: protein binding; protein serine/threonine kinase activity
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Research Articles on PBK/TOPK

1. findings suggest that PBK/TOPK plays a crucial role in tumor malignant potential through its overexpression in ESCC

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PBK/TOPK antibody

 Reference Product  PubMed Publications
 MELK antibody  >2 publications with PBK/TOPK and MELK
 NUSAP1 antibody  >1 publications with PBK/TOPK and NUSAP1
 BUB1 antibody  >1 publications with PBK/TOPK and BUB1
 TP53 antibody  >1 publications with PBK/TOPK and TP53
 CCNA2 antibody  >1 publications with PBK/TOPK and CCNA2
 CDK1 antibody  >1 publications with PBK/TOPK and CDK1

Diseases associated with anti-PBK/TOPK antibody

 Disease Name  Pubmed Publications
 Edema Antibodies  >24 publications with PBK/TOPK and Edema
 Nervous System Diseases Antibodies  >21 publications with PBK/TOPK and Nervous System Diseases
 Adenocarcinoma Antibodies  >11 publications with PBK/TOPK and Adenocarcinoma
 Liver Diseases Antibodies  >9 publications with PBK/TOPK and Liver Diseases
 Breast Neoplasms Antibodies  >8 publications with PBK/TOPK and Breast Neoplasms
 Inflammation Antibodies  >8 publications with PBK/TOPK and Inflammation
 Disease Models, Animal Antibodies  >7 publications with PBK/TOPK and Disease Models, Animal
 Liver Neoplasms Antibodies  >6 publications with PBK/TOPK and Liver Neoplasms
 Hypertension Antibodies  >6 publications with PBK/TOPK and Hypertension
 Lung Diseases Antibodies  >6 publications with PBK/TOPK and Lung Diseases

Organs/Tissues associated with anti-PBK/TOPK antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >60 publications with PBK/TOPK and Eye
 Blood Antibodies  >32 publications with PBK/TOPK and Blood
 Liver Antibodies  >25 publications with PBK/TOPK and Liver
 Muscle Antibodies  >18 publications with PBK/TOPK and Muscle
 Kidney Antibodies  >15 publications with PBK/TOPK and Kidney
 Brain Antibodies  >14 publications with PBK/TOPK and Brain
 Lung Antibodies  >11 publications with PBK/TOPK and Lung
 Embryonic Tissue Antibodies  >8 publications with PBK/TOPK and Embryonic Tissue
 Skin Antibodies  >7 publications with PBK/TOPK and Skin
 Vascular Antibodies  >7 publications with PBK/TOPK and Vascular
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