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GCNF Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476329

Entrez Summary: This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind… More details

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Product Overview

Online Datasheet in PDF
Product Name
GCNF, Monoclonal Antibody
Full Product Name
Anti-GCNF Mouse mAb
Product Gene Name
anti-GCNF antibody
Product Synonym Gene Name
NR6A1; RTR; GCNF; NR61; GCNF1
Product Synonym Names
GCNF 1; GCNF; GCNF1; Germ cell nuclear factor; hGCNF; hRTR; NR 61; NR61; nr6a1; NR6A1_HUMAN; Nuclear receptor subfamily 6 group A member 1; Orphan nuclear receptor NR6A1; Retinoic acid receptor related testis associated receptor; Retinoid receptor related testis specific receptor; Retinoid receptor-related testis-specific receptor; RTR
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B11-D3-F11
Host
Mouse
Species Reactivity
Human
Form/Format
Purified antibody in PBS with 0.05% sodium azide
Molecular Weight
54kDa
OMIM
S83309 mRNA
3D Structure
ModBase 3D Structure for Q15406
Immunogen
Purified recombinant fragment of human NR6A1 (AA: 65-118) expressed in E Coli.
Sequence Similarities
Contains 1 nuclear receptor DNA-binding domain.
Subunit Structure
Homodimer. Interacts with UIMC1 (By similarity).
Subcellular Location
Nucleus
Applications Tested/Suitable for anti-GCNF antibody
Western Blot (WB), Immunofluorescence (IF)

Western Blot (WB) of anti-GCNF antibody

Western blot detection of GCNF in Jurkat, MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.
Mouse Anti-GCNF Monoclonal Antibody – Cat# MBS476329

Immunocytochemistry (ICC) of anti-GCNF antibody

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-GCNF mouse mAb (dilution 1:200).
Mouse Anti-GCNF Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS476329

Western Blot (WB) of anti-GCNF antibody

Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.
Mouse Anti-GCNF Monoclonal Antibody, Western Blot (WB) – Cat# MBS476329

Immunofluorescence (IF) of anti-GCNF antibody

Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue
Mouse Anti-GCNF Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS476329

Flow Cytometry (FC/FACS) of anti-GCNF antibody

Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).
Mouse Anti-GCNF Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476329

Immunohistochemistry (IHC) of anti-GCNF antibody

Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.
Mouse Anti-GCNF Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476329

Immunohistochemistry (IHC) of anti-GCNF antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.
Mouse Anti-GCNF Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476329

Other Notes

Small volumes of anti-GCNF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCNF purchase

MBS476329 is an antibody targeting GCNF. Also known as Anti-GCNF Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GCNF. It may not necessarily be applicable to this product.

NCBI GI #
513788273
NCBI GeneID
2649
NCBI Accession #
NP_001265475.1
UniProt Primary Accession #
Q15406
UniProt Secondary Accession #
O00551; O00603; Q5T6F4; Q8NHQ0; Q92898; Q99802
UniProt Related Accession #
Q15406
GenBank Nucleotide #
NM_001278546.1
NCBI Official Full Name
nuclear receptor subfamily 6 group A member 1 isoform 4
NCBI Official Synonym Full Names
nuclear receptor subfamily 6 group A member 1
NCBI Official Symbol
NR6A1
NCBI Official Synonym Symbols
RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
UniProt Protein Name
Nuclear receptor subfamily 6 group A member 1
UniProt Synonym Protein Names
Germ cell nuclear factor; GCNF; hGCNF; Retinoid receptor-related testis-specific receptor; RTR; hRTR
Protein Family
Nuclear receptor subfamily
UniProt Gene Name
NR6A1
UniProt Synonym Gene Names
GCNF; GCNF; hGCNF; RTR; hRTR
NCBI Protein Information
nuclear receptor subfamily 6 group A member 1

NCBI Summary for GCNF

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]
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UniProt Comments for GCNF

NR6A1: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. Homodimer. Interacts with UIMC1. Shows highest expression in the germ cells of the adult testis. Belongs to the nuclear hormone receptor family. NR6 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; sequence-specific DNA binding

Biological Process: cell proliferation; gamete generation; negative regulation of transcription from RNA polymerase II promoter; spermatogenesis; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter
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Research Articles on GCNF

1. the genes, and up-regulated 64% in undifferentiated hES cells. In addition, GCNF also showed a regulatory gene pattern that is different from RA treatment during hES cell differentiation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GCNF antibody

 Reference Product  PubMed Publications
 NCOR1 antibody  >2 publications with GCNF and NCOR1

Pathways associated with anti-GCNF antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram

Diseases associated with anti-GCNF antibody

 Disease Name  Pubmed Publications
 Gonadal Disorders Antibodies  >4 publications with GCNF and Gonadal Disorders
 Congenital Abnormalities Antibodies  >3 publications with GCNF and Congenital Abnormalities
 Urogenital Abnormalities Antibodies  >2 publications with GCNF and Urogenital Abnormalities
 Amenorrhea Antibodies  >1 publications with GCNF and Amenorrhea
 Liver Diseases Antibodies  >1 publications with GCNF and Liver Diseases
 Infertility, Female Antibodies  >1 publications with GCNF and Infertility, Female
 Nervous System Malformations Antibodies  >1 publications with GCNF and Nervous System Malformations
 Abnormalities, Drug-Induced Antibodies  >1 publications with GCNF and Abnormalities, Drug-Induced

Organs/Tissues associated with anti-GCNF antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >32 publications with GCNF and Testis
 Ovary Antibodies  >14 publications with GCNF and Ovary
 Bone Antibodies  >8 publications with GCNF and Bone
 Placenta Antibodies  >3 publications with GCNF and Placenta
 Blood Antibodies  >2 publications with GCNF and Blood
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