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ENO2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476331

Entrez Summary: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during… More details

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Product Overview

Online Datasheet in PDF
Product Name
ENO2, Monoclonal Antibody
Full Product Name
Anti-ENO2 Mouse mAb
Product Gene Name
Product Synonym Gene Name
NSE
Product Synonym Names
NSE
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D3
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Molecular Weight
47.3
OMIM
X13120 mRNA
Immunogen
Purified recombinant fragment of human ENO2 (AA: 251-433) expressed in E Coli.
Applications Tested/Suitable for anti-ENO2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-ENO2 antibody

Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)
Mouse Anti-ENO2 Monoclonal Antibody – Cat# MBS476331

Western Blot (WB) of anti-ENO2 antibody

Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.
Mouse Anti-ENO2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476331

Flow Cytometry (FC/FACS) of anti-ENO2 antibody

Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).
Mouse Anti-ENO2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476331

Immunohistochemistry (IHC) of anti-ENO2 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.
Mouse Anti-ENO2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476331

Immunohistochemistry (IHC) of anti-ENO2 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.
Mouse Anti-ENO2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476331

Other Notes

Small volumes of anti-ENO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO2 purchase

MBS476331 is an antibody targeting ENO2. Also known as Anti-ENO2 Mouse mAb. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
ENO2
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
NCBI Protein Information
gamma-enolase

NCBI Summary for ENO2

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

UniProt Comments for ENO2

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular space

Molecular Function: phosphopyruvate hydratase activity; protein binding

Biological Process: gluconeogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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